Cloned (Comment) | Organism |
---|---|
heterologously expressed in Pichia pastoris. Recombinant enzyme production on the 5-L bioreactor scale exceeds the productivity of the wild type strain by a factor of 800 | Rhizoctonia solani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0183 | - |
chlorogenic acid | pH 6.0, 37°C | Rhizoctonia solani | |
0.0285 | - |
methyl caffeate | pH 6.0, 37°C | Rhizoctonia solani | |
0.0358 | - |
methyl p-coumarate | pH 6.0, 37°C | Rhizoctonia solani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhizoctonia solani | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizoctonia solani | A0A1D0BQV8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhizoctonia solani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | submerged cultures of Rhizoctonia solani grown in the presence of sugar beet pectin or Tween 80 | Rhizoctonia solani | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorogenic acid + H2O | - |
Rhizoctonia solani | ? | - |
? | |
ethyl cinnamate + H2O | - |
Rhizoctonia solani | ? | - |
? | |
ethyl ferulate + H2O | - |
Rhizoctonia solani | ? | - |
? | |
methyl caffeate + H2O | - |
Rhizoctonia solani | ? | - |
? | |
methyl cinnamate + H2O | - |
Rhizoctonia solani | ? | - |
? | |
methyl ferulate + H2O | - |
Rhizoctonia solani | ? | - |
? | |
methyl p-coumarate + H2O | - |
Rhizoctonia solani | ? | - |
? | |
methyl sinapate + H2O | - |
Rhizoctonia solani | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAE3 | - |
Rhizoctonia solani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhizoctonia solani |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 20°C: about 75% of maximal activity, 40°C: about 60% of maximal activity. The enzyme still possesses more than 80% residual activity at 37°C, activity decreases drastically above 40°C, reaching a plateau of approximately 10% activity between 50°C and 90°C | Rhizoctonia solani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, 55% residual activity | Rhizoctonia solani |
40 | - |
1 h, activity is almost lost | Rhizoctonia solani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
methyl caffeate | pH 6.0, 37°C | Rhizoctonia solani | |
29.3 | - |
chlorogenic acid | pH 6.0, 37°C | Rhizoctonia solani | |
33.1 | - |
methyl p-coumarate | pH 6.0, 37°C | Rhizoctonia solani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
substrate: methyl ferulate | Rhizoctonia solani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | the enzyme retaines more than 50% residual activity in the pH range 4.0-7.0. At pH 7.5 less than 10% residual activity is detected, while no activity is found at pH 8.0, substrate: methyl ferulate | Rhizoctonia solani |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
701.8 | - |
methyl caffeate | pH 6.0, 37°C | Rhizoctonia solani | |
925.3 | - |
methyl p-coumarate | pH 6.0, 37°C | Rhizoctonia solani | |
1598.4 | - |
chlorogenic acid | pH 6.0, 37°C | Rhizoctonia solani |