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Literature summary for 3.1.1.B10 extracted from

  • Nieter, A.; Kelle, S.; Linke, D.; Berger, R.G.
    A p-coumaroyl esterase from Rhizoctonia solani with a pronounced chlorogenic acid esterase activity (2017), New Biotechnol., 37, 153-161 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Pichia pastoris. Recombinant enzyme production on the 5-L bioreactor scale exceeds the productivity of the wild type strain by a factor of 800 Rhizoctonia solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0183
-
chlorogenic acid pH 6.0, 37°C Rhizoctonia solani
0.0285
-
methyl caffeate pH 6.0, 37°C Rhizoctonia solani
0.0358
-
methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rhizoctonia solani
-
-

Organism

Organism UniProt Comment Textmining
Rhizoctonia solani A0A1D0BQV8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhizoctonia solani

Source Tissue

Source Tissue Comment Organism Textmining
additional information submerged cultures of Rhizoctonia solani grown in the presence of sugar beet pectin or Tween 80 Rhizoctonia solani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorogenic acid + H2O
-
Rhizoctonia solani ?
-
?
ethyl cinnamate + H2O
-
Rhizoctonia solani ?
-
?
ethyl ferulate + H2O
-
Rhizoctonia solani ?
-
?
methyl caffeate + H2O
-
Rhizoctonia solani ?
-
?
methyl cinnamate + H2O
-
Rhizoctonia solani ?
-
?
methyl ferulate + H2O
-
Rhizoctonia solani ?
-
?
methyl p-coumarate + H2O
-
Rhizoctonia solani ?
-
?
methyl sinapate + H2O
-
Rhizoctonia solani ?
-
?

Synonyms

Synonyms Comment Organism
FAE3
-
Rhizoctonia solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Rhizoctonia solani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 20°C: about 75% of maximal activity, 40°C: about 60% of maximal activity. The enzyme still possesses more than 80% residual activity at 37°C, activity decreases drastically above 40°C, reaching a plateau of approximately 10% activity between 50°C and 90°C Rhizoctonia solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
1 h, 55% residual activity Rhizoctonia solani
40
-
1 h, activity is almost lost Rhizoctonia solani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
methyl caffeate pH 6.0, 37°C Rhizoctonia solani
29.3
-
chlorogenic acid pH 6.0, 37°C Rhizoctonia solani
33.1
-
methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
substrate: methyl ferulate Rhizoctonia solani

pH Range

pH Minimum pH Maximum Comment Organism
4 7 the enzyme retaines more than 50% residual activity in the pH range 4.0-7.0. At pH 7.5 less than 10% residual activity is detected, while no activity is found at pH 8.0, substrate: methyl ferulate Rhizoctonia solani

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
701.8
-
methyl caffeate pH 6.0, 37°C Rhizoctonia solani
925.3
-
methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
1598.4
-
chlorogenic acid pH 6.0, 37°C Rhizoctonia solani