BRENDA - Enzyme Database
show all sequences of 3.1.1.96

GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase

Wydau, S.; Ferri-Fioni, M.L.; Blanquet, S.; Plateau, P.; Nucleic Acids Res. 35, 930-938 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0011
-
D-tyrosyl-tRNATyr
pH 7.8, 27C
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
zinc ions are essential to the activity of the protein
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34726
-
x * 34726, calculated from sequence
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9ZPQ3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-aspartyl-tRNAAsp + H2O
initial rate of hydrolysis is very close to that obtained with D-tyrosyl-tRNATyr
726062
Arabidopsis thaliana
D-aspartate + tRNAAsp
-
-
-
?
D-tyrosyl-tRNATyr + H2O
no activity with L-tyrosyl-tRNATyr and diacetyl-L-Lys-tRNALys
726062
Arabidopsis thaliana
D-tyrosine + tRNATyr
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34726, calculated from sequence
Arabidopsis thaliana
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
assay at
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
300
-
D-tyrosyl-tRNATyr
pH 7.8, 27C
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0011
-
D-tyrosyl-tRNATyr
pH 7.8, 27C
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
zinc ions are essential to the activity of the protein
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34726
-
x * 34726, calculated from sequence
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-aspartyl-tRNAAsp + H2O
initial rate of hydrolysis is very close to that obtained with D-tyrosyl-tRNATyr
726062
Arabidopsis thaliana
D-aspartate + tRNAAsp
-
-
-
?
D-tyrosyl-tRNATyr + H2O
no activity with L-tyrosyl-tRNATyr and diacetyl-L-Lys-tRNALys
726062
Arabidopsis thaliana
D-tyrosine + tRNATyr
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34726, calculated from sequence
Arabidopsis thaliana
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
assay at
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
300
-
D-tyrosyl-tRNATyr
pH 7.8, 27C
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
272700
-
D-tyrosyl-tRNATyr
pH 7.8, 27C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
272700
-
D-tyrosyl-tRNATyr
pH 7.8, 27C
Arabidopsis thaliana
Other publictions for EC 3.1.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750406
Pawar
Role of D-aminoacyl-tRNA deac ...
Escherichia coli
eLife
6
e24001
2017
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1
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4
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2
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2
2
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752311
Calendar
D-Tyrosyl-tRNA deacylase A ne ...
Escherichia coli
Trends Biochem. Sci.
42
684-686
2017
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1
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1
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1
1
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750073
Rybak
-
Cloning, expression and purif ...
Thermus thermophilus
Biopolymers Cell
31
179-186
2015
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1
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1
2
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2
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1
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2
2
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751492
Geraskina
The dtd gene from Bacillus am ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens A50
Microbiol. Res.
171
90-96
2015
-
1
1
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6
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4
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1
1
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724218
Zheng
Human D-Tyr-tRNA(Tyr) deacylas ...
Homo sapiens, Mus musculus
Biochem. J.
417
85-94
2009
-
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3
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5
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2
2
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725386
Wydau
Widespread distribution of cel ...
Synechocystis sp.
J. Biol. Chem.
284
14096-14104
2009
-
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1
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1
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1
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1
1
1
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6
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1
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1
1
1
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1
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1
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1
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1
1
1
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1
1
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1
1
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1
1
726062
Wydau
GEK1, a gene product of Arabid ...
Arabidopsis thaliana
Nucleic Acids Res.
35
930-938
2007
-
-
1
-
-
-
1
1
-
1
1
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3
-
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2
1
1
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1
1
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1
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1
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1
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1
1
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2
1
1
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1
1
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1
1
725371
Ferri-Fioni
Identification in archaea of a ...
Archaeoglobus fulgidus, Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543, Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
281
27575-27585
2006
1
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3
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10
-
2
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4
5
5
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12
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2
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2
10
2
2
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2
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3
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10
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5
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2
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2
10
2
2
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2
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663379
Rigden
Archaea recruited D-Tyr-tRNATy ...
Azotobacter vinelandii, Escherichia coli, Haemophilus influenzae
RNA
10
1845-1851
2004
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3
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7
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3
3
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3
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3
3
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729964
Soutourina
Formation of D-tyrosyl-tRNATyr ...
Escherichia coli, Escherichia coli K-37
J. Biol. Chem.
279
42560-42565
2004
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2
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2
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1
1
-
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725353
Lim
A catalytic mechanism for D-Ty ...
Haemophilus influenzae, Haemophilus influenzae DSM 11121
J. Biol. Chem.
278
13496-13502
2003
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1
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4
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1
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1
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1
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1
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1
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1
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1
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1
1
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729723
Yang
D-Amino acids and D-Tyr-tRNA(T ...
Escherichia coli
FEBS Lett.
552
95-98
2003
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1
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725346
Ferri-Fioni
Structure of crystalline D-Tyr ...
Escherichia coli
J. Biol. Chem.
276
47285-47290
2001
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1
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1
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3
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1
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1
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1
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1
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1
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1
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725339
Soutourina
Metabolism of D-aminoacyl-tRNA ...
Escherichia coli, Saccharomyces cerevisiae
J. Biol. Chem.
275
32535-32542
2000
-
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5
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1
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5
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1
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1
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1
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5
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1
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1
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2
2
725336
Soutourina
Functional characterization of ...
Escherichia coli
J. Biol. Chem.
274
19109-19114
1999
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1
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1
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2
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1
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8
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1
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2
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1
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1
1
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1
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1
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2
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1
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1
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2
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1
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1
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1
1