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Literature summary for 3.1.1.95 extracted from
- Modifications of aclacinomycin T by aclacinomycin methyl esterase (RdmC) and aclacinomycin-10-hydroxylase (RdmB) from Streptomyces purpurascens (2000), Biochim. Biophys. Acta, 1480, 191-200.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Streptomyces lividans TK24 | Streptomyces purpurascens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0155 | - |
aclacinomycin T | pH 7.5, 37°C | Streptomyces purpurascens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, SDS-PAGE | Streptomyces purpurascens |
| 31729 | - |
1 * 31729, calculated from sequence | Streptomyces purpurascens |
| 33000 | - |
gel filtration | Streptomyces purpurascens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aclacinomycin T + H2O | Streptomyces purpurascens | - |
15-demethylaclacinomycin T + methanol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces purpurascens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces purpurascens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Streptomyces purpurascens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 169.4 | - |
pH 7.5, 37°C | Streptomyces purpurascens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aclacinomycin T + H2O | - |
Streptomyces purpurascens | 15-demethylaclacinomycin T + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Streptomyces purpurascens |
| monomer | 1 * 31729, calculated from sequence | Streptomyces purpurascens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RdmC | - |
Streptomyces purpurascens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptomyces purpurascens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | 30°C: 58% of maximal activity, 45°C: 87% of maximal activity | Streptomyces purpurascens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces purpurascens |
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Release 2023.1 (January 2023)
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