Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.90 extracted from

  • Takahashi, Y.; Moiseyev, G.; Nikolaeva, O.; Ma, J.X.
    Identification of the key residues determining the product specificity of isomerohydrolase (2012), Biochemistry, 51, 4217-4225.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.90

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293A cells Danio rerio
expression of wild-type enzyme and mutants in HEK-293A cells Danio rerio

Protein Variants

Protein Variants Comment Organism
F103L in terms of isomerization specificity mutant shows a substantially increases level of production of 13-cis retinol Danio rerio
K222M in terms of isomerization specificity mutant only slightly increases level of production of 13-cis retinol Danio rerio
L103F site-directed mutagenesis, site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 13-cis-retinol to 11-cis-retinol, product of EC 3.1.1.64. Formation of 62.5% 13-cis-retinol and 37.5% 11-cis-retinol Danio rerio
L103F in terms of isomerization specificity mutant shows a highly reduced level of 13-cis retinol while increasing the level of 11-cis-retinol (compared to wild-tpye) Danio rerio
L133S in terms of isomerization specificity mutant shows a substantially increases level of production of 13-cis retinol Danio rerio
M222K site-directed mutagenesis, the mutation does not affect the enzyme isomerization product specificity of the enzyme Danio rerio
M222K in terms of isomerization specificity mutant shows no effect and generates exclusively 13-cis-retinol similar to wild-type Danio rerio
N58Y site-directed mutagenesis, the mutation completely reverses the enzyme isomerization product specificity from formation of 13-cis-retinol to 11-cis-retinol, product of EC 3.1.1.64. Formation of 28.7% 13-cis-retinol and 71.3% 11-cis-retinol Danio rerio
N58Y in terms of isomerization specificity mutant shows a highly reduced level of 13-cis retinol while increasing the level of 11-cis-retinol (compared to wild-tpye) Danio rerio
N58Y/L103F site-directed mutagenesis, the mutation does not affect the enzyme isomerization product specificity of the enzyme Danio rerio
N58Y/L103F N58Y/L103F double mutant shows a product specificity identical to that of wild-type 13cIMH Danio rerio
S133L site-directed mutagenesis, site-directed mutagenesis, the mutation reverses the enzyme isomerization product specificity from formation of 13-cis-retinol to 11-cis-retinol, product of EC 3.1.1.64. Formation of 94.4% 13-cis-retinol and 5.6% 11-cis-retinol Danio rerio
S133L in terms of isomerization specificity mutant shows only a slightly reduced level of 13-cis retinol while increasing the level of 11-cis-retinol (compared to wild-tpye) Danio rerio
Y58N in terms of isomerization specificity mutant generates exclusively 13-cis retinol Danio rerio
Y58N/F103L double mutant generates predominantly 11-cis retinol Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an all-trans-retinyl ester + H2O Danio rerio the enzyme generates exclusively 13-cis-retinol 13-cis-retinol + a fatty acid
-
 ?

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Danio rerio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans retinyl ester + H2O 13cIMH generates exclusively 13-cis retinol Danio rerio 13-cis retinol + ?
-
 ?
all-trans retinyl ester + H2O RPE65c generates predominatly 11-cis retinol and only little 13-cis retinol Danio rerio 11-cis retinol + ?
-
 ?
an all-trans-retinyl ester + H2O the enzyme generates exclusively 13-cis-retinol Danio rerio 13-cis-retinol + a fatty acid
-
 ?
an all-trans-retinyl ester + H2O the enzyme generates exclusively 13-cis-retinol, no formation of 11-cis-retinol Danio rerio 13-cis-retinol + a fatty acid
-
 ?

Synonyms

Synonyms Comment Organism
13-cis-isomerohydrolase
-
 Danio rerio
13-cis-specific isomerohydrolase
-
 Danio rerio
13cIMH
-
 Danio rerio
isomerohydrolase
-
 Danio rerio
RPE65b formerly Danio rerio
RPE65c
-
 Danio rerio

General Information

General Information Comment Organism
evolution it is likely that the two novel homologues of RPE65 (13cIMH and RPE65c, EC 3.1.1.90 and EC 3.1.1.64, respectively) are generated through gene duplication after the separation of fish RPE65 from the ancestral RPE65, because they exhibit an extremely high level of sequence identity (97%) and are located in the same chromosome, but on a different chromosome from RPE65 Danio rerio
additional information key residues determining the isomerization product specificity of the enzyme are Tyr58, Phe103, and Leu133 Danio rerio