Any feedback?
Literature summary for 3.1.1.88 extracted from
- Identification and immobilization of a novel cold-adapted esterase, and its potential for bioremediation of pyrethroid-contaminated vegetables (2017), Microb. Cell Fact., 16, 149 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | high potential for bioremediation of pyrethroid-contaminated vegetables | uncultured microorganism |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) in soluble form | uncultured microorganism |
General Stability
| General Stability | Organism |
|---|---|
| high reusability, the immobilized Est684 (immobilized on on mesoporous silica) retains nearly 54% of its activity after 28 cycles, indicating excellent operational stability | uncultured microorganism |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00601 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, soluble enzyme | uncultured microorganism |  |
| 0.00792 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, immobilized enzyme | uncultured microorganism | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
below, SDS-PAGE | uncultured microorganism |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured microorganism | A0A290U7B6 | gene (est684) isolated from the Mao-tofu metagenome | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
uncultured microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | about 70% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | highest activity towards 4-nitrophenyl butyrate among all tested 4-nitrophenyl esters | uncultured microorganism | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl decanoate + H2O | about 10% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl dodecanoate + H2O | about 5% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + dodecanoate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | about 85% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | about 35% of the activity with 4-nitrophenyl butyrate | uncultured microorganism | 4-nitrophenol + octanoate | - |
? | |
| cyhalothrin + H2O | - |
uncultured microorganism | 3-phenoxybenzaldehyde + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
| cypermethrin + H2O | - |
uncultured microorganism | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
| fenvalerate + H2O | - |
uncultured microorganism | 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | uncultured microorganism |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| est684 | - |
uncultured microorganism |
| pyrethroid-hydrolyzing esterase | - |
uncultured microorganism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | - |
soluble and immobilized enzyme | uncultured microorganism |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 45 | 5°C: about 60% of maximal activity, 45°C: about 50% of maximal activity, immobilized enzyme | uncultured microorganism |
| 8 | 40 | 8°C: about 55% of maximal activity, 40°C: about 40% of maximal activity, soluble enzyme | uncultured microorganism |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | - |
52% residual activity after incubation for 18 h | uncultured microorganism |
| 35 | - |
t1/2: 6 h ( immobilized enzyme), 1.5 h (soluble enzyme) | uncultured microorganism |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 403.47 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, immobilized enzyme | uncultured microorganism | |
| 417.15 | - |
4-nitrophenyl butyrate | pH 6.5, 18°C, soluble enzyme | uncultured microorganism | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | immobilized enzyme | uncultured microorganism |
| 7 | - |
soluble enzyme | uncultured microorganism |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | the relative activity of the immobilized enzyme is more than 75% at the pH range between 5.5 and 8.0, while the soluble enzyme attains activity over 50% at the same pH range | uncultured microorganism |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
