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Literature summary for 3.1.1.88 extracted from

  • Guo, P.; Wang, B.; Hang, B.; Li, L.; Ali, W.; He, J.; Li, S.
    Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase (2009), Int. Biodeter. Biodegrad., 63, 1107-1112.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ more than 75% enzyme activity inhibition Sphingobium sp.
Cu2+ more than 75% enzyme activity inhibition Sphingobium sp.
Hg2+ more than 75% enzyme activity inhibition Sphingobium sp.
malathion 0.5 mM, 96% inhibition Sphingobium sp.
p-chloromercuribenzoic acid 0.5 mM, complete inhibition Sphingobium sp.
phenylmethylsulfonyl fluoride 0.5 mM, 99% inhibition Sphingobium sp.
SDS 10 mM, 90% inhibition Sphingobium sp.
Zn2+ more than 75% enzyme activity inhibition Sphingobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
gel filtration Sphingobium sp.

Organism

Organism UniProt Comment Textmining
Sphingobium sp.
-
-
-
Sphingobium sp. JZ-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphingobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.042
-
pH and temperature not specified in the publication Sphingobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bifenthrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. (2-methylbiphenyl-3-yl)methanol + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate
-
?
bifenthrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. JZ-2 (2-methylbiphenyl-3-yl)methanol + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate
-
?
cypermethrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
?
cypermethrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. JZ-2 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
?
deltamethrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
?
deltamethrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. JZ-2 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
?
fenpropathrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide cyano-3-phenoxybenzyl alcohol is unstable and is transformed spontaneously to 3-phenoxybenzaldehyde ?
fenpropathrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. JZ-2 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide cyano-3-phenoxybenzyl alcohol is unstable and is transformed spontaneously to 3-phenoxybenzaldehyde ?
fenvalerate + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide
-
?
fenvalerate + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. JZ-2 3-phenoxybenzaldehyde + 2-(4-chlorophenyl)-3-methylbutanoic acid + cyanide
-
?
permethrin + H2O fenpropathrin is the most preferred substrate. Whereas the hydrolysis rates toward fenpropathrin, permethrin and cypermethrin are not significantly different, fenvalerate, deltamethrin and bifenthrin are degraded at a considerable lower rate than fenpropathrin Sphingobium sp. 3-phenoxybenzyl alcohol + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Sphingobium sp.

Synonyms

Synonyms Comment Organism
pyrethroid-hydrolyzing enzyme
-
Sphingobium sp.
pytH
-
Sphingobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Sphingobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
fairly stable below Sphingobium sp.
65
-
nearly completely inactivated Sphingobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Sphingobium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8 more than 75% of maximal activity at pH 6.0 and at pH 8.0 Sphingobium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 stable Sphingobium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors or coenzymes are required for the pyrethroid-hydrolysis activity Sphingobium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Sphingobium sp. isoelectric focusing, pH-range: 3.5-10
-
4.85