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Literature summary for 3.1.1.85 extracted from

  • Sanishvili, R.; Yakunin, A.F.; Laskowski, R.A.; Skarina, T.; Evdokimova, E.; Doherty-Kirby, A.; Lajoie, G.A.; Thornton, J.M.; Arrowsmith, C.H.; Savchenko, A.; Joachimiak, A.; Edwards, A.M.
    Integrating structure, bioinformatics, and enzymology to discover function. BioH, a new carboxylesterase from Escherichia coli (2003), J. Biol. Chem., 278, 26039-26045.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the Escherichia coli auxotroph strain B834 (DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.2 M sodium citrate trihydrate and 0.1 M Tris-HCl (pH 8.0), at 21°C Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
phenylmethylsulfonyl fluoride 10.5% residual activity after 10 min of incubation with 2 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 4-nitrophenyl caproate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
0.29
-
 4-nitrophenyl acetate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
0.33
-
 4-nitrophenyl butyrate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
0.35
-
 4-nitrophenyl propionate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
0.6
-
 4-nitrophenyl laurate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P13001
-
-
Escherichia coli DH5-alpha P13001
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O highest carboxylesterase activity Escherichia coli ?
-
 ?
4-nitrophenyl butyrate + H2O
-
 Escherichia coli ?
-
 ?
4-nitrophenyl caprate + H2O
-
 Escherichia coli ?
-
 ?
4-nitrophenyl caproate + H2O
-
 Escherichia coli ?
-
 ?
4-nitrophenyl laurate + H2O
-
 Escherichia coli ?
-
 ?
4-nitrophenyl palmitate + H2O
-
 Escherichia coli ?
-
 ?
4-nitrophenyl propionate + H2O
-
 Escherichia coli ?
-
 ?
4-nitrophenyl stearate + H2O very low carboxylesterase activity Escherichia coli ?
-
 ?
additional information purified BioH shows low enzymatic activities for thioesterase (using palmitoyl-CoA as a substrate), lipase (using olive oil as a substrate), and aminopeptidase (using leucine-4-anilide as a substrate) and shows no detectable enzymatic activity for phosphatase (using 4-nitrophenyl phosphate as a substrate), trypsin-like endopeptidase (using benzoyl-arginine-4-nitroanilide as a substrate), or bromoperoxidase (phenol red and monochlorodimedone as potential substrates) Escherichia coli ?
-
 ?
pimelyl-[acyl-carrier protein] methyl ester + H2O
-
 Escherichia coli pimelyl-[acyl-carrier protein] + methanol
-
 ?

Synonyms

Synonyms Comment Organism
BioH
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 4-nitrophenyl laurate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
4
-
 4-nitrophenyl caproate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
6.1
-
 4-nitrophenyl butyrate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
13.1
-
 4-nitrophenyl propionate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
18.5
-
 4-nitrophenyl acetate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism BioH is involved in biotin biosynthesis Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
 4-nitrophenyl laurate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
16
-
 4-nitrophenyl caproate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
18.5
-
 4-nitrophenyl butyrate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
37.4
-
 4-nitrophenyl propionate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli
63.8
-
 4-nitrophenyl acetate in 50 mM HEPES-K (pH 7.5) buffer, at 37°C Escherichia coli