Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Rhodococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
LMWP-S-S-T172R/G173Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Rhodococcus sp. |
LMWP-T172R/G173Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Rhodococcus sp. |
T172R/G173Q -LMWP | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Rhodococcus sp. |
T172R/G173Q -YGRKKRRQRRR | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Rhodococcus sp. |
YGRKKRRQRRR-T172R/G173Q | the mutant still maintains 52% of its cocaine-hydrolyzing efficiency even after incubation at 37°C for 24 h | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0288 | - |
cocaine | wild type enzyme, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0305 | - |
cocaine | mutant enzyme LMWP-S-S-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0306 | - |
cocaine | mutant enzyme T172R/G173Q -YGRKKRRQRRR, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0396 | - |
cocaine | mutant enzyme YGRKKRRQRRR-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.0415 | - |
cocaine | mutant enzyme T172R/G173Q -LMWP, at pH 7.4 and 37°C | Rhodococcus sp. | |
0.049 | - |
cocaine | mutant enzyme LMWP-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cocaine + H2O | Rhodococcus sp. | - |
ecgonine methyl ester + benzoate | - |
? | |
cocaine + H2O | Rhodococcus sp. MB1 | - |
ecgonine methyl ester + benzoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | Q9L9D7 | - |
- |
Rhodococcus sp. MB1 | Q9L9D7 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity column chromatography and Q-Sepharose column chromatography | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cocaine + H2O | - |
Rhodococcus sp. | ecgonine methyl ester + benzoate | - |
? | |
cocaine + H2O | - |
Rhodococcus sp. MB1 | ecgonine methyl ester + benzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Rhodococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
cocE | - |
Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the in vitro half-life at 37°C is about 13.7 min | Rhodococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.45 | - |
cocaine | mutant enzyme T172R/G173Q -YGRKKRRQRRR, at pH 7.4 and 37°C | Rhodococcus sp. | |
32.62 | - |
cocaine | mutant enzyme YGRKKRRQRRR-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
34.42 | - |
cocaine | mutant enzyme LMWP-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
35.17 | - |
cocaine | mutant enzyme T172R/G173Q -LMWP, at pH 7.4 and 37°C | Rhodococcus sp. | |
39.17 | - |
cocaine | mutant enzyme LMWP-S-S-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
44.85 | - |
cocaine | wild type enzyme, at pH 7.4 and 37°C | Rhodococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
702 | - |
cocaine | mutant enzyme LMWP-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
823 | - |
cocaine | mutant enzyme YGRKKRRQRRR-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
847 | - |
cocaine | mutant enzyme T172R/G173Q -LMWP, at pH 7.4 and 37°C | Rhodococcus sp. | |
1028 | - |
cocaine | mutant enzyme T172R/G173Q -YGRKKRRQRRR, at pH 7.4 and 37°C | Rhodococcus sp. | |
1283 | - |
cocaine | mutant enzyme LMWP-S-S-T172R/G173Q, at pH 7.4 and 37°C | Rhodococcus sp. | |
1560 | - |
cocaine | wild type enzyme, at pH 7.4 and 37°C | Rhodococcus sp. |