BRENDA - Enzyme Database show
show all sequences of 3.1.1.84

A thermally stable form of bacterial cocaine esterase: a potential therapeutic agent for treatment of cocaine abuse

Brim, R.L.; Nance, M.R.; Youngstrom, D.W.; Narasimhan, D.; Zhan, C.G.; Tesmer, J.J.; Sunahara, R.K.; Woods, J.H.; Mol. Pharmacol. 77, 593-600 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
the wild type cocaine esterase cannot be used as a pharmacotherapy for cocaine abuse because of its 13.7-min half-life at 37°C
Rhodococcus sp.
Crystallization (Commentary)
Crystallization
Organism
mutant enzyme L169K/G173Q, hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 10 mM Tris-HCl, pH 7.3, and 25 mM NaCl
Rhodococcus sp.
Engineering
Amino acid exchange
Commentary
Organism
L169K/G173Q
highly thermostable mutant with a half-life of 2.9 days at 37°C
Rhodococcus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0072
-
cocaine
wild type enzyme, without preincubation, at pH 7.4 and 37°C
Rhodococcus sp.
0.0073
-
cocaine
wild type enzyme, after 24 h preincubation at 37°C, at pH 7.4 and 37°C
Rhodococcus sp.
0.0229
-
cocaine
mutant enzyme L169K/G173Q, after 24 h preincubation at 37°C, at pH 7.4 and 37°C
Rhodococcus sp.
0.0303
-
cocaine
mutant enzyme L169K/G173Q, without preincubation, at pH 7.4 and 37°C
Rhodococcus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus sp.
Q9L9D7
-
-
Rhodococcus sp. MB1
Q9L9D7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cocaine + H2O
-
716276
Rhodococcus sp.
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
-
716276
Rhodococcus sp. MB1
ecgonine methyl ester + benzoate
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
the wild type enzyme shows 10.8% residual activity after 24 h at 37°C, mutant enzyme L169K/G173Q shows 77.5% residual activity after 24 h at 37°C
Rhodococcus sp.
Application (protein specific)
Application
Commentary
Organism
medicine
the wild type cocaine esterase cannot be used as a pharmacotherapy for cocaine abuse because of its 13.7-min half-life at 37°C
Rhodococcus sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
mutant enzyme L169K/G173Q, hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 10 mM Tris-HCl, pH 7.3, and 25 mM NaCl
Rhodococcus sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L169K/G173Q
highly thermostable mutant with a half-life of 2.9 days at 37°C
Rhodococcus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0072
-
cocaine
wild type enzyme, without preincubation, at pH 7.4 and 37°C
Rhodococcus sp.
0.0073
-
cocaine
wild type enzyme, after 24 h preincubation at 37°C, at pH 7.4 and 37°C
Rhodococcus sp.
0.0229
-
cocaine
mutant enzyme L169K/G173Q, after 24 h preincubation at 37°C, at pH 7.4 and 37°C
Rhodococcus sp.
0.0303
-
cocaine
mutant enzyme L169K/G173Q, without preincubation, at pH 7.4 and 37°C
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cocaine + H2O
-
716276
Rhodococcus sp.
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
-
716276
Rhodococcus sp. MB1
ecgonine methyl ester + benzoate
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
the wild type enzyme shows 10.8% residual activity after 24 h at 37°C, mutant enzyme L169K/G173Q shows 77.5% residual activity after 24 h at 37°C
Rhodococcus sp.
Other publictions for EC 3.1.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750291
Kim
Kinetic characterization of c ...
Homo sapiens
Chem. Biol. Interact.
293
107-114
2018
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1
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3
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1
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3
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5
-
1
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3
1
-
-
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1
1
-
3
3
728856
Fang
Rational design, preparation, ...
Rhodococcus sp., Rhodococcus sp. MB1 Bresler
ACS Chem. Biol.
9
1764-1772
2014
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1
1
3
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3
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2
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3
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2
1
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3
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3
3
730250
Brim
The fate of bacterial cocaine ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
340
83-95
2012
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1
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1
1
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-
-
-
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-
730421
Lee
Cell permeable cocaine esteras ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharm.
9
1361-1373
2012
-
-
1
-
5
-
-
6
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1
2
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6
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1
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2
1
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1
6
-
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1
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5
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6
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1
2
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1
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2
1
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1
6
-
-
-
-
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-
-
-
6
6
716278
Xue
Design, preparation, and chara ...
Homo sapiens
Mol. Pharmacol.
79
290-297
2011
-
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1
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3
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4
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1
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1
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1
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1
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4
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1
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3
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4
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1
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1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
716281
Narasimhan
Subunit stabilization and pegy ...
Rhodococcus sp.
Mol. Pharmacol.
80
1056-1065
2011
-
-
1
1
3
-
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4
-
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1
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3
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1
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1
1
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1
4
-
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1
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1
3
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4
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1
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1
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1
1
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1
4
-
-
-
-
-
-
-
-
4
4
714226
Zheng
Design of high-activity mutant ...
Homo sapiens
Biochemistry
49
9113-9119
2010
-
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1
1
10
-
-
-
-
-
-
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-
1
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1
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1
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1
10
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1
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9
9
714702
Yang
Characterization of a high-act ...
Homo sapiens
Chem. Biol. Interact.
187
148-152
2010
-
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1
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2
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1
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2
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2
2
716276
Brim
A thermally stable form of bac ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharmacol.
77
593-600
2010
-
1
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1
1
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-
4
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7
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2
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2
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1
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-
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-
716824
Narasimhan
Structural analysis of thermos ...
Rhodococcus sp., Rhodococcus sp. MB1
Protein Eng.
23
537-547
2010
-
1
1
-
5
-
-
4
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6
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1
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2
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1
5
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1
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1
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2
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1
5
-
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5
5
692028
Ko
Effects of cocaine esterase fo ...
Rhodococcus sp.
Drug Alcohol Depend.
101
202-209
2009
-
1
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3
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-
694267
Gao
Thermostable variants of cocai ...
Rhodococcus sp.
Mol. Pharmacol.
75
318-323
2009
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1
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4
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3
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1
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1
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1
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4
1
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699760
Collins
Zhan. C.G.; Woods. J.H.: Cocai ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
331
445-455
2009
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1
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1
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4
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-
698483
Liu
Fundamental reaction mechanism ...
Rhodococcus sp.
J. Am. Chem. Soc.
131
11964-11975
2003
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650108
Turner
Biochemical characterization a ...
Rhodococcus sp.
Biochemistry
41
12297-12307
2002
-
1
1
1
11
-
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10
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1
1
2
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3
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9
1
1
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1
1
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1
11
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10
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1
1
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3
-
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9
1
1
-
-
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-
-
-
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-
653322
Larsen
Crystal structure of a bacteri ...
Rhodococcus sp.
Nat. Struct. Biol.
9
17-21
2002
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1
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1
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695729
Bresler
Gene cloning and nucleotide se ...
Rhodococcus sp., Rhodococcus sp. MB1
Appl. Environ. Microbiol.
66
904-908
2000
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1
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1
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2
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11
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1
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1
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6
1
1
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1
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-
171019
Pindel
Purification and cloning of a ...
Homo sapiens
J. Biol. Chem.
272
14769-14775
1997
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1
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4
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1
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1
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5
1
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-
698540
Britt
Identification of a cocaine es ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia MB11L
J. Bacteriol.
174
2087-2094
1992
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2
3
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3
2
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2
1
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1
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