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Literature summary for 3.1.1.84 extracted from

  • Yang, W.; Xue, L.; Fang, L.; Chen, X.; Zhan, C.
    Characterization of a high-activity mutant of human butyrylcholinesterase against (-)-cocaine (2010), Chem. Biol. Interact., 187, 148-152.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.84

Protein Variants

Protein Variants Comment Organism
A199S/S287G/A328W/Y332G the mutant shows high activity towards (-)-cocaine with about 1080fold (un-fused) and 100fold (when fused with human serum albumin) improved catalytic efficiency compared to the wild type enzyme and also leads to a decrease in catalytic efficiency with acetylthiocholine and butyrylthiocholine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 (-)-cocaine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
0.0045
-
 (-)-cocaine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-cocaine + H2O
-
 Homo sapiens benzoic acid + methyl-(1R,2R,3S,5S)-3-hydroxy-8-methyl-8-azabicyclo[3.2.1]-octane-2-carboxylate i.e. ecgonine methyl ester ?

Synonyms

Synonyms Comment Organism
BChE
-
 Homo sapiens
butyrylcholinesterase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.068
-
 (-)-cocaine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
51
-
 (-)-cocaine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.17
-
 (-)-cocaine wild type enzyme, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens
16500
-
 (-)-cocaine mutant enzyme A199S/S287G/A328W/Y332G, at 25°C, in 0.1 M potassium phosphate, pH 7.5 Homo sapiens