BRENDA - Enzyme Database show
show all sequences of 3.1.1.84

Characterization of a high-activity mutant of human butyrylcholinesterase against (-)-cocaine

Yang, W.; Xue, L.; Fang, L.; Chen, X.; Zhan, C.; Chem. Biol. Interact. 187, 148-152 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
A199S/S287G/A328W/Y332G
the mutant shows high activity towards (-)-cocaine with about 1080fold (un-fused) and 100fold (when fused with human serum albumin) improved catalytic efficiency compared to the wild type enzyme and also leads to a decrease in catalytic efficiency with acetylthiocholine and butyrylthiocholine
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0031
-
(-)-cocaine
mutant enzyme A199S/S287G/A328W/Y332G, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
0.0045
-
(-)-cocaine
wild type enzyme, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-cocaine + H2O
-
714702
Homo sapiens
benzoic acid + methyl-(1R,2R,3S,5S)-3-hydroxy-8-methyl-8-azabicyclo[3.2.1]-octane-2-carboxylate
i.e. ecgonine methyl ester
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.068
-
(-)-cocaine
wild type enzyme, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
51
-
(-)-cocaine
mutant enzyme A199S/S287G/A328W/Y332G, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A199S/S287G/A328W/Y332G
the mutant shows high activity towards (-)-cocaine with about 1080fold (un-fused) and 100fold (when fused with human serum albumin) improved catalytic efficiency compared to the wild type enzyme and also leads to a decrease in catalytic efficiency with acetylthiocholine and butyrylthiocholine
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0031
-
(-)-cocaine
mutant enzyme A199S/S287G/A328W/Y332G, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
0.0045
-
(-)-cocaine
wild type enzyme, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-cocaine + H2O
-
714702
Homo sapiens
benzoic acid + methyl-(1R,2R,3S,5S)-3-hydroxy-8-methyl-8-azabicyclo[3.2.1]-octane-2-carboxylate
i.e. ecgonine methyl ester
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.068
-
(-)-cocaine
wild type enzyme, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
51
-
(-)-cocaine
mutant enzyme A199S/S287G/A328W/Y332G, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
15.17
-
(-)-cocaine
wild type enzyme, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
16500
-
(-)-cocaine
mutant enzyme A199S/S287G/A328W/Y332G, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
15.17
-
(-)-cocaine
wild type enzyme, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
16500
-
(-)-cocaine
mutant enzyme A199S/S287G/A328W/Y332G, at 25C, in 0.1 M potassium phosphate, pH 7.5
Homo sapiens
Other publictions for EC 3.1.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750291
Kim
Kinetic characterization of c ...
Homo sapiens
Chem. Biol. Interact.
293
107-114
2018
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5
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3
1
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1
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3
3
728856
Fang
Rational design, preparation, ...
Rhodococcus sp., Rhodococcus sp. MB1 Bresler
ACS Chem. Biol.
9
1764-1772
2014
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1
1
3
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3
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3
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2
1
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3
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3
3
730250
Brim
The fate of bacterial cocaine ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
340
83-95
2012
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1
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1
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730421
Lee
Cell permeable cocaine esteras ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharm.
9
1361-1373
2012
-
-
1
-
5
-
-
6
-
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1
2
-
6
-
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1
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2
1
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1
6
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5
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6
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2
1
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1
6
-
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6
6
716278
Xue
Design, preparation, and chara ...
Homo sapiens
Mol. Pharmacol.
79
290-297
2011
-
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1
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3
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4
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1
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1
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4
-
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4
4
716281
Narasimhan
Subunit stabilization and pegy ...
Rhodococcus sp.
Mol. Pharmacol.
80
1056-1065
2011
-
-
1
1
3
-
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4
-
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1
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3
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1
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1
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4
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4
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4
4
714226
Zheng
Design of high-activity mutant ...
Homo sapiens
Biochemistry
49
9113-9119
2010
-
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1
1
10
-
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1
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1
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10
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1
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9
9
714702
Yang
Characterization of a high-act ...
Homo sapiens
Chem. Biol. Interact.
187
148-152
2010
-
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1
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2
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2
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2
2
716276
Brim
A thermally stable form of bac ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharmacol.
77
593-600
2010
-
1
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1
1
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4
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7
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716824
Narasimhan
Structural analysis of thermos ...
Rhodococcus sp., Rhodococcus sp. MB1
Protein Eng.
23
537-547
2010
-
1
1
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5
-
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4
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6
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1
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1
5
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5
5
692028
Ko
Effects of cocaine esterase fo ...
Rhodococcus sp.
Drug Alcohol Depend.
101
202-209
2009
-
1
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694267
Gao
Thermostable variants of cocai ...
Rhodococcus sp.
Mol. Pharmacol.
75
318-323
2009
-
1
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4
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3
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1
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1
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1
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699760
Collins
Zhan. C.G.; Woods. J.H.: Cocai ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
331
445-455
2009
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1
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698483
Liu
Fundamental reaction mechanism ...
Rhodococcus sp.
J. Am. Chem. Soc.
131
11964-11975
2003
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650108
Turner
Biochemical characterization a ...
Rhodococcus sp.
Biochemistry
41
12297-12307
2002
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1
1
1
11
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10
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1
1
2
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9
1
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11
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1
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653322
Larsen
Crystal structure of a bacteri ...
Rhodococcus sp.
Nat. Struct. Biol.
9
17-21
2002
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695729
Bresler
Gene cloning and nucleotide se ...
Rhodococcus sp., Rhodococcus sp. MB1
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66
904-908
2000
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171019
Pindel
Purification and cloning of a ...
Homo sapiens
J. Biol. Chem.
272
14769-14775
1997
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698540
Britt
Identification of a cocaine es ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia MB11L
J. Bacteriol.
174
2087-2094
1992
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