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Literature summary for 3.1.1.81 extracted from

  • See-Too, W.S.; Convey, P.; Pearce, D.A.; Chan, K.G.
    Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp (2018), Microb. Cell Fact., 17, 179 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 StarTM cells Planococcus versutus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, about 30% inhibition, in vivo and in vitro assay Planococcus versutus
Cr3+ 1 mM, 30% inhibition in the in vitro assay, about 20% inhibition in the in vivo assay Planococcus versutus
Cu2+ 1 mM, 45% inhibition in the in vitro assay, about 50% inhibition in the in vivo assay Planococcus versutus
EDTA
-
 Planococcus versutus
Fe2+ 1 mM, about 30% inhibition, in vivo and in vitro assay Planococcus versutus
Mg2+ 1 mM, 90% inhibition in the in vitro assay, about 30% inhibition in the in vivo assay Planococcus versutus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 1 mM, 1.45fold activation in the in vivo assay Planococcus versutus

Organism

Organism UniProt Comment Textmining
Planococcus versutus
-
-
-
Planococcus versutus L10.15T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Planococcus versutus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxododecanoyl-L-homoserine lactone + H2O about 60% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O about 60% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O best substrate Planococcus versutus N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O best substrate Planococcus versutus L10.15T N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxooctanoyl-L-homoserine lactone + H2O about 80% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-3-oxooctanoyl-L-homoserine
-
 ?
N-3-oxooctanoyl-L-homoserine lactone + H2O about 80% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-3-oxooctanoyl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O about 10% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-butyryl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O about 40% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O about 40% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-decanoyl-L-homoserine
-
 ?
N-heptanoyl-L-homoserine lactone + H2O about 10% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-heptanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O about 90% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O about 90% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus L10.15T N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O about 55% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone Planococcus versutus N-octanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AHL lactonase
-
 Planococcus versutus
AidP
-
 Planococcus versutus
N-acylhomoserine lactonase
-
 Planococcus versutus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Planococcus versutus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
17 43 17°C: about 60% of maximal activity, 43°C: about 40% of maximal activity Planococcus versutus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32
-
 3 h, about 35% loss of activity Planococcus versutus
37
-
 3 h, about 75% loss of activity Planococcus versutus
40
-
 3 h, about 80% loss of activity Planococcus versutus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Planococcus versutus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 50% of maximal activity, pH 8.0-9.0: maximal activity Planococcus versutus