Application | Comment | Organism |
---|---|---|
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition | Sphingopyxis sp. EG6 |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition | Sphingopyxis sp. FD7 |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition | Sphingopyxis bauzanensis |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition | Sphingomonas ursincola |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggested that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition | Sphingopyxis alaskensis |
Cloned (Comment) | Organism |
---|---|
heterologous expression in Escherichia coli DH5alpha | Sphingopyxis sp. FD7 |
heterologous expression in Escherichia coli DH5alpha | Sphingopyxis alaskensis |
heterologous expression in Escherichia coli DH5alpha | Sphingopyxis bauzanensis |
heterologous expression in Escherichia coli DH5alpha | Sphingomonas ursincola |
heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation | Sphingopyxis sp. EG6 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Sphingopyxis sp. EG6 | - |
- |
extracellular | - |
Sphingopyxis sp. FD7 | - |
- |
extracellular | - |
Sphingomonas ursincola | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Sphingopyxis sp. EG6 |
30000 | - |
SDS-PAGE | Sphingopyxis sp. FD7 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas ursincola | - |
- |
- |
Sphingopyxis alaskensis | - |
- |
- |
Sphingopyxis alaskensis DSM 13593 | - |
- |
- |
Sphingopyxis bauzanensis | - |
- |
- |
Sphingopyxis bauzanensis DSM 22271 | - |
- |
- |
Sphingopyxis sp. EG6 | A0A2Z5U8E0 | isolated from industrial cooling water samples | - |
Sphingopyxis sp. FD7 | A0A2Z5UR29 | isolated from industrial cooling water samples | - |
Purification (Comment) | Organism |
---|---|
- |
Sphingopyxis sp. EG6 |
His-tagged enzyme | Sphingopyxis sp. FD7 |
His-tagged enzyme | Sphingopyxis alaskensis |
His-tagged enzyme | Sphingopyxis bauzanensis |
His-tagged enzyme | Sphingomonas ursincola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. EG6 | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. FD7 | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingomonas ursincola | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis DSM 22271 | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis DSM 13593 | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. EG6 | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. FD7 | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingomonas ursincola | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis DSM 22271 | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis DSM 13593 | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. EG6 | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. FD7 | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingomonas ursincola | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis DSM 22271 | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis DSM 13593 | N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. EG6 | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. FD7 | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingomonas ursincola | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis bauzanensis DSM 22271 | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis alaskensis DSM 13593 | N-hexanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. EG6 | N-octanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | - |
Sphingopyxis sp. FD7 | N-octanoyl-L-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Sphingopyxis sp. EG6 |
? | x * 30000, SDS-PAGE | Sphingopyxis sp. FD7 |
Synonyms | Comment | Organism |
---|---|---|
AHL lactonase | - |
Sphingopyxis sp. EG6 |
AHL lactonase | - |
Sphingopyxis sp. FD7 |
AHL lactonase | - |
Sphingopyxis alaskensis |
AHL lactonase | - |
Sphingopyxis bauzanensis |
AHL lactonase | - |
Sphingomonas ursincola |
qsdS | - |
Sphingopyxis sp. EG6 |
qsdS | - |
Sphingopyxis sp. FD7 |
qsdS | - |
Sphingopyxis alaskensis |
qsdS | - |
Sphingopyxis bauzanensis |
qsdS | - |
Sphingomonas ursincola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
substrate: N-octanoyl-L-homoserine lactone | Sphingopyxis sp. EG6 |
60 | 70 | substrate: N-octanoyl-L-homoserine lactone | Sphingopyxis sp. FD7 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C: about 40% of maximal activity, 80°C: about 35% of maximal activity, substrate: N-octanoyl-L-homoserine lactone | Sphingopyxis sp. EG6 |
50 | 80 | 50°C: about 65% of maximal activity, 80°C: about 80% of maximal activity, substrate: N-octanoyl-L-homoserine lactone | Sphingopyxis sp. FD7 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, stable | Sphingopyxis sp. FD7 |
50 | - |
5 min, stable | Sphingopyxis sp. EG6 |
50 | - |
5 min, 10% loss of activity | Sphingopyxis sp. FD7 |
60 | - |
5 min, 80% loss of activity | Sphingopyxis sp. FD7 |
60 | - |
5 min, about 10% loss of activity | Sphingopyxis sp. EG6 |
70 | - |
5 min, complete loss of activity | Sphingopyxis sp. FD7 |
70 | - |
5 min, about 55% loss of activity | Sphingopyxis sp. EG6 |
80 | - |
5 min, complete inactivation | Sphingopyxis sp. EG6 |
General Information | Comment | Organism |
---|---|---|
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation | Sphingopyxis sp. EG6 |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation | Sphingopyxis sp. FD7 |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation | Sphingopyxis alaskensis |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation | Sphingopyxis bauzanensis |
additional information | heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation | Sphingomonas ursincola |