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Literature summary for 3.1.1.81 extracted from

  • Morohoshi, T.; Kamimura, Y.; Sato, N.; Iizumi, T.
    Distribution and characterization of N-acylhomoserine lactone (AHL)-degrading activity and AHL lactonase gene (qsdS) in Sphingopyxis (2018), J. Biosci. Bioeng., 127, 411-417 .
    View publication on PubMed

Application

Application Comment Organism
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis sp. EG6
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis sp. FD7
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis bauzanensis
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggest that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingomonas ursincola
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation. These results suggested that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition Sphingopyxis alaskensis

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli DH5alpha Sphingopyxis sp. FD7
heterologous expression in Escherichia coli DH5alpha Sphingopyxis alaskensis
heterologous expression in Escherichia coli DH5alpha Sphingopyxis bauzanensis
heterologous expression in Escherichia coli DH5alpha Sphingomonas ursincola
heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis sp. EG6

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Sphingopyxis sp. EG6
-
-
extracellular
-
Sphingopyxis sp. FD7
-
-
extracellular
-
Sphingomonas ursincola
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
SDS-PAGE Sphingopyxis sp. EG6
30000
-
SDS-PAGE Sphingopyxis sp. FD7

Organism

Organism UniProt Comment Textmining
Sphingomonas ursincola
-
-
-
Sphingopyxis alaskensis
-
-
-
Sphingopyxis alaskensis DSM 13593
-
-
-
Sphingopyxis bauzanensis
-
-
-
Sphingopyxis bauzanensis DSM 22271
-
-
-
Sphingopyxis sp. EG6 A0A2Z5U8E0 isolated from industrial cooling water samples
-
Sphingopyxis sp. FD7 A0A2Z5UR29 isolated from industrial cooling water samples
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphingopyxis sp. EG6
His-tagged enzyme Sphingopyxis sp. FD7
His-tagged enzyme Sphingopyxis alaskensis
His-tagged enzyme Sphingopyxis bauzanensis
His-tagged enzyme Sphingomonas ursincola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. EG6 N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. FD7 N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingomonas ursincola N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis DSM 22271 N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis DSM 13593 N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. EG6 N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. FD7 N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingomonas ursincola N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis DSM 22271 N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis DSM 13593 N-3-oxohexanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. EG6 N-decanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. FD7 N-decanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis N-decanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis N-decanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingomonas ursincola N-decanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis DSM 22271 N-decanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis DSM 13593 N-decanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. EG6 N-hexanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. FD7 N-hexanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis N-hexanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis N-hexanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingomonas ursincola N-hexanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis bauzanensis DSM 22271 N-hexanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Sphingopyxis alaskensis DSM 13593 N-hexanoyl-L-homoserine
-
?
N-octanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. EG6 N-octanoyl-L-homoserine
-
?
N-octanoyl-L-homoserine lactone + H2O
-
Sphingopyxis sp. FD7 N-octanoyl-L-homoserine
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Sphingopyxis sp. EG6
? x * 30000, SDS-PAGE Sphingopyxis sp. FD7

Synonyms

Synonyms Comment Organism
AHL lactonase
-
Sphingopyxis sp. EG6
AHL lactonase
-
Sphingopyxis sp. FD7
AHL lactonase
-
Sphingopyxis alaskensis
AHL lactonase
-
Sphingopyxis bauzanensis
AHL lactonase
-
Sphingomonas ursincola
qsdS
-
Sphingopyxis sp. EG6
qsdS
-
Sphingopyxis sp. FD7
qsdS
-
Sphingopyxis alaskensis
qsdS
-
Sphingopyxis bauzanensis
qsdS
-
Sphingomonas ursincola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. EG6
60 70 substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. FD7

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 40% of maximal activity, 80°C: about 35% of maximal activity, substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. EG6
50 80 50°C: about 65% of maximal activity, 80°C: about 80% of maximal activity, substrate: N-octanoyl-L-homoserine lactone Sphingopyxis sp. FD7

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, stable Sphingopyxis sp. FD7
50
-
5 min, stable Sphingopyxis sp. EG6
50
-
5 min, 10% loss of activity Sphingopyxis sp. FD7
60
-
5 min, 80% loss of activity Sphingopyxis sp. FD7
60
-
5 min, about 10% loss of activity Sphingopyxis sp. EG6
70
-
5 min, complete loss of activity Sphingopyxis sp. FD7
70
-
5 min, about 55% loss of activity Sphingopyxis sp. EG6
80
-
5 min, complete inactivation Sphingopyxis sp. EG6

General Information

General Information Comment Organism
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis sp. EG6
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis sp. FD7
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis alaskensis
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingopyxis bauzanensis
additional information heterologous expression of qsdS in Pseudomonas aeruginosa results in reduction of biofilm formation Sphingomonas ursincola