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Literature summary for 3.1.1.81 extracted from

  • Ghani, N.; Sulaiman, J.; Ismail, Z.; Chan, X.; Yin, W.; Chan, K.
    Rhodotorula Mucilaginosa, a quorum quenching yeast exhibiting lactonase activity isolated from a tropical shoreline (2014), Sensors, 14, 6463-6473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Rhodotorula mucilaginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of AidH at 1.29 A resolution and the structures of AidH-C4-HS (N-butanoyl-L-homoserine), mutant S102G AidH-C6-HSL (N-hexanoyl-L-homoserine lactone) and mutant E219G AidH-C6-HS (N-hexanoyl-L-homoserine) complexes at resolutions of 1.09, 1.33 and 1.11 A, respectively, are determined Rhodotorula mucilaginosa

Organism

Organism UniProt Comment Textmining
Rhodotorula mucilaginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Rhodotorula mucilaginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-hydroxyhexanoyl)-L-homoserine lactone + H2O
-
Rhodotorula mucilaginosa N-(3-hydroxyhexanoyl)-L-homoserine
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
-
Rhodotorula mucilaginosa N-(3-oxo-N-hexanoyl)-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O
-
Rhodotorula mucilaginosa N-hexanoyl-L-homoserine
-
?

Synonyms

Synonyms Comment Organism
AHL-lactonase
-
Rhodotorula mucilaginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Rhodotorula mucilaginosa