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Literature summary for 3.1.1.81 extracted from

  • Hanano, A.; Harba, M.; Al-Ali, M.; Ammouneh, H.
    Silencing of Erwinia amylovora sy69 AHL-quorum sensing by a Bacillus simplex AHL-inducible aiiA gene encoding a zinc-dependent N-acyl-homoserine lactonase (2014), Plant Pathol., 63, 773-783.
    View publication on PubMed
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Peribacillus simplex

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Peribacillus simplex

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 SDS-PAGE Peribacillus simplex

Organism

Organism UniProt Comment Textmining
Peribacillus simplex
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Peribacillus simplex

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O relative activity: 97.2% Peribacillus simplex N-(3-oxo-N-hexanoyl)-L-homoserine
-
 ?
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O relative activity: 100% Peribacillus simplex N-(3-oxo-octanoyl)-L-homoserine
-
 ?
N-butanoyl-L-homoserine lactone + H2O weak substrate Peribacillus simplex N-butanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Peribacillus simplex N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O relative activity: 98.9% Peribacillus simplex N-octanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AHL-lactonase
-
 Peribacillus simplex
AiiA
-
 Peribacillus simplex

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Peribacillus simplex

Expression

Organism Comment Expression
Peribacillus simplex expression of the aiiA gene is significantly up-regulated by 3-oxo-AHL up

General Information

General Information Comment Organism
physiological function AHL-lactonase inhibits multiplication of the N-(3-oxo-N-hexanoyl) L-homoserine lactone-producing plant pathogen Erwinia amylovora sy69 both in vitro and in planta Peribacillus simplex