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Literature summary for 3.1.1.81 extracted from
- Enzymatic hydrolysis of molecules associated with bacterial quorum sensing using an acyl homoserine lactonase from a novel Bacillus thuringiensis strain (2014), Antonie van Leeuwenhoek, 105, 253-264.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacillus thuringiensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |  |
| 0.86 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 3.81 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 8.84 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | strongly inhibits enzymatic activity | Bacillus thuringiensis | |
| Cu2+ | abolishes enzymatic activity | Bacillus thuringiensis | |
| Fe2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
| K+ | weakly inhibits eznymatic activity | Bacillus thuringiensis | |
| Mg2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
| Mn2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
| Pb2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Bacillus thuringiensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Bacillus thuringiensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-butanoyl-DL-homoserine lactone + H2O | relative activity: 190% | Bacillus thuringiensis | N-butanoyl-DL-homoserine | - |
? | |
| N-heptanoyl-DL-homoserine lactone + H2O | relative activity: 73% | Bacillus thuringiensis | N-heptanoyl-DL-homoserine | - |
? | |
| N-hexanoyl-DL-homoserine lactone + H2O | relative activity: 100% | Bacillus thuringiensis | N-hexanoyl-DL-homoserine | - |
? | |
| N-octanoyl-DL-homoserine lactone + H2O | relative activity: 457% | Bacillus thuringiensis | N-octanoyl-DL-homoserine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AHL | - |
Bacillus thuringiensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacillus thuringiensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | enzyme shows reduced activity at 70°C | Bacillus thuringiensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Bacillus thuringiensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00549 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 0.0102 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 0.0235 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 0.0446 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus thuringiensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | reduced activity at pH 6 | Bacillus thuringiensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00266 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 0.0109 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 0.0117 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
| 0.0119 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
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