Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.86 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
3.81 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
8.84 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | strongly inhibits enzymatic activity | Bacillus thuringiensis | |
Cu2+ | abolishes enzymatic activity | Bacillus thuringiensis | |
Fe2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
K+ | weakly inhibits eznymatic activity | Bacillus thuringiensis | |
Mg2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
Mn2+ | inhibits enzymatic activity | Bacillus thuringiensis | |
Pb2+ | inhibits enzymatic activity | Bacillus thuringiensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-butanoyl-DL-homoserine lactone + H2O | relative activity: 190% | Bacillus thuringiensis | N-butanoyl-DL-homoserine | - |
? | |
N-heptanoyl-DL-homoserine lactone + H2O | relative activity: 73% | Bacillus thuringiensis | N-heptanoyl-DL-homoserine | - |
? | |
N-hexanoyl-DL-homoserine lactone + H2O | relative activity: 100% | Bacillus thuringiensis | N-hexanoyl-DL-homoserine | - |
? | |
N-octanoyl-DL-homoserine lactone + H2O | relative activity: 457% | Bacillus thuringiensis | N-octanoyl-DL-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus thuringiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | enzyme shows reduced activity at 70°C | Bacillus thuringiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00549 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.0102 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.0235 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.0446 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | reduced activity at pH 6 | Bacillus thuringiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00266 | - |
N-heptanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.0109 | - |
N-butanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.0117 | - |
N-octanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis | |
0.0119 | - |
N-hexanoyl-DL-homoserine lactone | pH 7.4, 32°C | Bacillus thuringiensis |