Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.81 extracted from

  • Gao, A.; Mei, G.Y.; Liu, S.; Wang, P.; Tang, Q.; Liu, Y.P.; Wen, H.; An, X.M.; Zhang, L.Q.; Yan, X.X.; Liang, D.C.
    High-resolution structures of AidH complexes provide insights into a novel catalytic mechanism for N-acyl homoserine lactonase (2013), Acta Crystallogr. Sect. D, 69, 82-91.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Ochrobactrum sp. T63

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of AidH at 1.29 A resolution and the structures of AidH-C4-HS (N-butanoyl homoserine), mutant S102G AidH-C6-HSL (N-hexanoyl homoserine lactone) and mutant E219G AidH-C6-HS (N-hexanoyl homoserine) complexes at resolutions of 1.09, 1.33 and 1.11 A, respectively, are determined Ochrobactrum sp. T63

Protein Variants

Protein Variants Comment Organism
E219G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 9.1%, activity with substrate N-dodecanoyl-L-homoserine lactone: 25.7%, crystal structure determined at 1.33 A Ochrobactrum sp. T63
F138G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 97.3%, activity with substrate N-dodecanoyl-L-homoserine lactone: 100% Ochrobactrum sp. T63
F189G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 88.8%, activity with substrate N-dodecanoyl-L-homoserine lactone: 86% Ochrobactrum sp. T63
F192G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 77.8%, activity with substrate N-dodecanoyl-L-homoserine lactone: 71.7% Ochrobactrum sp. T63
F221G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 72.7%, activity with substrate N-dodecanoyl-L-homoserine lactone: 77.8% Ochrobactrum sp. T63
H248G mutant shows no activity Ochrobactrum sp. T63
L103G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 77.2%, activity with substrate N-dodecanoyl-L-homoserine lactone: 78.5% Ochrobactrum sp. T63
M144G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 98.7%, activity with substrate N-dodecanoyl-L-homoserine lactone: 82.2% Ochrobactrum sp. T63
M188G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 99.2%, activity with substrate N-dodecanoyl-L-homoserine lactone: 68.2% Ochrobactrum sp. T63
M77G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 92.2%, activity with substrate N-dodecanoyl-L-homoserine lactone: 80.4% Ochrobactrum sp. T63
N33G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 96.5%, activity with substrate N-dodecanoyl-L-homoserine lactone: 92.1% Ochrobactrum sp. T63
S102G mutant shows no activity, crystal structure determined at 1.33 A Ochrobactrum sp. T63
Y160G activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: no activity, activity with substrate N-dodecanoyl-L-homoserine lactone: 10.3% Ochrobactrum sp. T63

Organism

Organism UniProt Comment Textmining
Ochrobactrum sp. T63 D2J2T6
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Ochrobactrum sp. T63

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-hydroxy-dodecanoyl)-L-homoserine lactone + H2O relative activity: 91.9% Ochrobactrum sp. T63 N-(3-hydroxy-dodecanoyl)-L-homoserine
-
 ?
N-(3-oxo-decanoyl)-L-homoserine lactone + H2O relative activity: 97.3% Ochrobactrum sp. T63 N-(3-oxo-decanoyl)-L-homoserine
-
 ?
N-(3-oxo-dodecanoyl)-L-homoserine lactone + H2O relative activity: 83.3% Ochrobactrum sp. T63 N-(3-oxo-dodecanoyl)-L-homoserine
-
 ?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O relative activity: 97.2% Ochrobactrum sp. T63 N-(3-oxo-N-hexanoyl)-L-homoserine
-
 ?
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O relative activity: 100% Ochrobactrum sp. T63 N-(3-oxo-octanoyl)-L-homoserine
-
 ?
N-(3-oxo-tetradecanoyl)-L-homoserine lactone + H2O relative activity: 95.4% Ochrobactrum sp. T63 N-(3-oxo-tetradecanoyl)-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O relative activity: 95.6% Ochrobactrum sp. T63 N-decanoyl-L-homoserine
-
 ?
N-dodecanoyl-L-homoserine lactone + H2O relative activity: 89.7% Ochrobactrum sp. T63 N-dodecanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O relative activity: 99.6% Ochrobactrum sp. T63 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O relative activity: 98.9% Ochrobactrum sp. T63 N-octanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AidH
-
 Ochrobactrum sp. T63

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ochrobactrum sp. T63

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Ochrobactrum sp. T63