Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus kaustophilus |
expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Geobacillus kaustophilus |
Crystallization (Comment) | Organism |
---|---|
D266N mutant, sitting drop vapor diffusion method, using 100 mM Tris, pH 7.5, 20% (w/v) PEG 4000, and 1.0 mM ZnCl2 | Geobacillus kaustophilus |
purified mutant D266N in complex with N-butyryl-L-homoserine lactone, sitting-drop vapor diffusion method, equal volumes of 45 mg/ml protein in 100 mM NaCl, 20 mM Tris-HCl, pH 8.0, and 1 mM N-butyryl-L-homoserine lactone, are mixed with precipitation solution containing 100 mM Tris, pH 7.5, 20% w/v PEG 4000, and 1.0 mM ZnCl2, 3 days, 20°C, X-ray diffraction structure determination and analysis at 1.6 A resolution | Geobacillus kaustophilus |
Protein Variants | Comment | Organism |
---|---|---|
D266N | inactive | Geobacillus kaustophilus |
D266N | catalytically inactive mutant | Geobacillus kaustophilus |
E101G/R230C | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Geobacillus kaustophilus |
E101G/R230C | the mutant has an increased value of kcat/Km of 72fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone | Geobacillus kaustophilus |
E101K/R230P | the mutant shows decreased catalytic efficiency toward 3-oxo-N-octanoyl-L-homoserine lactone and no activioty towards 3-oxo-N-hexanoyl-L-homoserine lactone compared to the wild type enzyme | Geobacillus kaustophilus |
E101N | inactive | Geobacillus kaustophilus |
E101N/R230I | the mutant has an increased value of kcat/Km of 72fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone | Geobacillus kaustophilus |
E101N/R230I | the mutant has an 72fold increased kcat/Km value toward 3-oxo-N-dodecanoyl-L-homoserine lactone compared to the wild type enzyme and also exhibits lactonase activity toward N-butyryl-L-homoserine lactone | Geobacillus kaustophilus |
E101V/R230T | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Geobacillus kaustophilus |
R230D | inactive | Geobacillus kaustophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.024 | - |
gamma-nonalactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.035 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.035 | - |
delta-nonalactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.095 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.095 | - |
N-octanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.13 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.13 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.13 | - |
N-decanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.13 | - |
N-decanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.39 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.39 | - |
N-octanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.46 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.46 | - |
gamma-nonalactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.48 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.48 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.69 | - |
paraoxon | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.74 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.74 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.1 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
1.1 | - |
gamma-nonalactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.1 | - |
delta-nonalactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.2 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
1.2 | - |
N-octanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2.4 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
2.4 | - |
N-hexanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2.6 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
2.6 | - |
N-hexanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2.9 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
2.9 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2.9 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
3.7 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
3.7 | - |
N-hexanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
3.8 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
3.8 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
5.9 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
5.9 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
9 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
9 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
9 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
26 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
26 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains iron | Geobacillus kaustophilus | |
Fe2+ | bound to wild-type as well as mutant enzymes | Geobacillus kaustophilus | |
Zn2+ | bound to wild-type as well as mutant enzymes | Geobacillus kaustophilus | |
Zn2+ | contains zinc, required for activity | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography and Q-Sepharose column chromatography | Geobacillus kaustophilus |
recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin, followed by anion exchange chromatography, to homogeneity | Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
delta-nonalactone + H2O | - |
Geobacillus kaustophilus | 5-hydroxynonanoate | - |
? | |
delta-nonalactone + H2O | high activity | Geobacillus kaustophilus | 5-hydroxynonanoate | - |
? | |
gamma-nonalactone + H2O | - |
Geobacillus kaustophilus | 4-hydroxynonanoate | - |
? | |
gamma-nonalactone + H2O | high activity | Geobacillus kaustophilus | 4-hydroxynonanoate | - |
? | |
additional information | the wild-type enzyme does also show low hydrolysis activity with paraoxon, while its mutants do not | Geobacillus kaustophilus | ? | - |
? | |
additional information | no activity towards N-butyryl-L-homoserine lactone, N-decanoyl-L-homoserine lactone, and N-(3-oxohexanoyl)-L-homoserine lactone | Geobacillus kaustophilus | ? | - |
? | |
N-(3-oxododecanoyl)-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-(3-oxododecanoyl)-L-homoserine | - |
? | |
N-(3-oxohexanoyl)-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-(3-oxohexanoyl)-L-homoserine | - |
? | |
N-(3-oxooctanoyl)-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-(3-oxooctanoyl)-L-homoserine | - |
? | |
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-3-oxo-dodecanoyl-L-homoserine | - |
? | |
N-3-oxo-hexanoyl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-3-oxo-hexanoyl-L-homoserine | - |
? | |
N-3-oxo-octanoyl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-3-oxo-octanoyl-L-homoserine | - |
? | |
N-butyryl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-butyryl-L-homoserine | - |
? | |
N-butyryl-L-homoserine lactone + H2O | substrate of mutant E101N/R230I, not of the wild-type enzyme | Geobacillus kaustophilus | N-butyryl-L-homoserine | - |
? | |
N-butyryl-L-homoserine lactone + H2O | substrate for mutant enzymes E101G/R230C and E101N/R230I | Geobacillus kaustophilus | N-butyryl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-DL-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-hexanoyl-L-homoserine | - |
? | |
N-octanoyl-DL-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-octanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | - |
Geobacillus kaustophilus | N-octanoyl-L-homoserine | - |
? | |
paraoxon + H2O | low activity | Geobacillus kaustophilus | diethylphosphate + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GKL | - |
Geobacillus kaustophilus |
More | the enzyme belongs to the phosphotriesterase-like lactonase group of enzymes within the amidohydrolase superfamily | Geobacillus kaustophilus |
N-acylhomoserine lactonase | - |
Geobacillus kaustophilus |
quorum-quenching lactonase | - |
Geobacillus kaustophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Geobacillus kaustophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the wild type enzyme retains its catalytic activity even after exposure to 60°C for 72 h | Geobacillus kaustophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
paraoxon | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.009 | - |
N-butyryl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.009 | - |
N-butyryl-L-homoserine lactone | kcat below 0.009 s-1, mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.011 | - |
N-butyryl-L-homoserine lactone | kcat below 0.011 s-1, mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.17 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.17 | - |
N-hexanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.2 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.2 | - |
N-hexanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.25 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.25 | - |
N-hexanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.29 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.29 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.3 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.3 | - |
N-octanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.4 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.4 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.4 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.43 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.43 | - |
N-decanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.52 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.52 | - |
N-octanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.91 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.91 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.1 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
1.1 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.4 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
1.4 | - |
N-octanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.6 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
1.6 | - |
delta-nonalactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.7 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
1.7 | - |
N-decanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.8 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
1.8 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.8 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
2 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
2 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2 | - |
gamma-nonalactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
2.9 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
2.9 | - |
gamma-nonalactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
6.9 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
6.9 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
22 | - |
gamma-nonalactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
29 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
29 | - |
delta-nonalactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Geobacillus kaustophilus |
General Information | Comment | Organism |
---|---|---|
evolution | directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily | Geobacillus kaustophilus |
additional information | a charge transfer complex involving the beta-cation and Tyr99 within the enzyme active site determines the coloration of the organism. Modulation of the charge transfer complex alters the lactonase activity of the mutant enzymes and is reflected in enzyme coloration changes | Geobacillus kaustophilus |
physiological function | the enzyme hydrolyze N-acylhomoserine lactones that are involved in virulence pathways of quorum-sensing pathogenic bacteria | Geobacillus kaustophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.0045 | - |
paraoxon | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.0045 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.017 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101V/R230T, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.021 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101K/R230P, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.035 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.035 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.045 | - |
N-3-oxo-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.045 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.045 | - |
N-(3-oxohexanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.05 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.05 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.066 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.066 | - |
N-hexanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.068 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.068 | - |
N-hexanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.082 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.082 | - |
N-hexanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.11 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101V/R230T, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.13 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.13 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.28 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
0.28 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.3 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.3 | - |
N-decanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.43 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
0.43 | - |
N-octanoyl-L-homoserine lactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.64 | - |
N-3-oxo-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
0.64 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
0.64 | - |
N-(3-oxooctanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
1.4 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
1.4 | - |
delta-nonalactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
3.2 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
3.2 | - |
N-octanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
3.3 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
3.3 | - |
N-decanoyl-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
3.7 | - |
N-octanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
3.7 | - |
N-octanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
3.9 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
3.9 | - |
delta-nonalactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
4.2 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
4.2 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
4.3 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
4.3 | - |
gamma-nonalactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
9.3 | - |
N-3-oxo-dodecanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
9.3 | - |
N-(3-oxododecanoyl)-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
13 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 37°C, recombinant mutant E101N/R230I | Geobacillus kaustophilus | |
13 | - |
N-decanoyl-L-homoserine lactone | mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
21 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant mutant E101G/R230C | Geobacillus kaustophilus | |
21 | - |
gamma-nonalactone | mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
120 | - |
gamma-nonalactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
120 | - |
gamma-nonalactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus | |
840 | - |
delta-nonalactone | pH 8.3, 37°C, recombinant wild-type enzyme | Geobacillus kaustophilus | |
840 | - |
delta-nonalactone | wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C | Geobacillus kaustophilus |