Cloned (Comment) | Organism |
---|---|
gene aiiA, DNA and amino acid sequence determination and analysis, expression of GST-tagged AiiA in Escherichia coli strain DH5alpha, expression in Erwinia carotovora strain SCG1 leading to reduction of autoinducer release thereby cedreasing extracellular pectolytic enzyme activities, and attenuating pathogenicity on potato, eggplant, Chinese cabbage, carrot, celery, cauliflower, and tobacco | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
D108E | site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant shows 90.8% of wild-type enzyme activity | Bacillus sp. (in: Bacteria) |
D108S | site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant | Bacillus sp. (in: Bacteria) |
D108S/H109S | site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant | Bacillus sp. (in: Bacteria) |
H104L/H106L/D108L | site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 61.4% of wild-type enzyme activity | Bacillus sp. (in: Bacteria) |
H104L/H106L/D108L/H109L | site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 37.9% of wild-type enzyme activity | Bacillus sp. (in: Bacteria) |
H104S | site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant is as active as the wild-type enzyme | Bacillus sp. (in: Bacteria) |
H104S/H106S | site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 51.1% of wild-type enzyme activity | Bacillus sp. (in: Bacteria) |
H104S/H106S/D108S/H109Sc | site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant | Bacillus sp. (in: Bacteria) |
H104S/H106S/H109S | site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant | Bacillus sp. (in: Bacteria) |
H106S | site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant shows 61.4% of wild-type enzyme activity | Bacillus sp. (in: Bacteria) |
H109S | site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metallohydrolase containing the conserved HXHXDH zinc-binding motif required for full enzyme activity | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acyl-(S)-homoserine lactone + H2O | Bacillus sp. (in: Bacteria) | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Bacillus sp. (in: Bacteria) 240B1 | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism | N-acyl-(S)-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q9L8R8 | isolated from soil, gene aiiA | - |
Bacillus sp. (in: Bacteria) 240B1 | Q9L8R8 | isolated from soil, gene aiiA | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged AiiA from Escherichia coli strain DH5alpha | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-3-oxodecanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-oxodecanoyl-L-homoserine | - |
? | |
N-3-oxodecanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) 240B1 | N-3-oxodecanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) 240B1 | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxooctanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-oxooctanoyl-L-homoserine | - |
? | |
N-3-oxooctanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) 240B1 | N-3-oxooctanoyl-L-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism | Bacillus sp. (in: Bacteria) | N-acyl-(S)-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism | Bacillus sp. (in: Bacteria) 240B1 | N-acyl-(S)-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AiiA | - |
Bacillus sp. (in: Bacteria) |