Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.81 extracted from

  • Thomas, P.W.; Stone, E.M.; Costello, A.L.; Tierney, D.L.; Fast, W.
    The quorum-quenching lactonase from Bacillus thuringiensis is a metalloprotein (2005), Biochemistry, 44, 7559-7569.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain DH5alpha Bacillus thuringiensis serovar kurstaki
gene aiiA, expression in Escherichia coli strain DH5alpha fused to the maltose binding protein with the TEV protease cleavage site ENLYFQ*G Bacillus thuringiensis serovar kurstaki

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, different enzyme constitutions Bacillus thuringiensis serovar kurstaki
6.7
-
N-hexanoyl-(S)-homoserine lactone pH 7.4, 28°C, purified detagged recombinant enzyme Bacillus thuringiensis serovar kurstaki

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dinuclear form, 2 Zn2+ ions per enzyme molecule, preparation of the apoenzyme, which is inactive, and reconstitution with Zn2+ at pH 7.1 restoring about 60% of native enzyme activity, metal content determination, overview Bacillus thuringiensis serovar kurstaki
Zn2+ the enzyme is a metalloprotein Bacillus thuringiensis serovar kurstaki

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, SDS-PAGE Bacillus thuringiensis serovar kurstaki
29000
-
x * 29000, recombinant detagged enzyme, SDS-PAGE Bacillus thuringiensis serovar kurstaki

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus thuringiensis serovar kurstaki N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence ?
-
?
additional information Bacillus thuringiensis serovar kurstaki 4A3 N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence ?
-
?
additional information Bacillus thuringiensis serovar kurstaki BGSC 4A3 N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis serovar kurstaki P0CJ63
-
-
Bacillus thuringiensis serovar kurstaki P0CJ63 gene aiiA
-
Bacillus thuringiensis serovar kurstaki 4A3 P0CJ63
-
-
Bacillus thuringiensis serovar kurstaki BGSC 4A3 P0CJ63 gene aiiA
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus thuringiensis serovar kurstaki
recombinant maltose binding protein-fusion enzyme from Escherichia coli strain DH5alpha to over 95% purity, the fusion tag is cleaved off by TEV protease, the detagged enzyme is purified by DEAE ion exchange chromatography, and amylose affinity chromatography to remove traces of MBP, further purification by hydrophobic interaction chromatography in presence of 1.6-2.0 M ammonium sulfate, and dialysis Bacillus thuringiensis serovar kurstaki

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus thuringiensis serovar kurstaki

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence Bacillus thuringiensis serovar kurstaki ?
-
?
additional information N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence Bacillus thuringiensis serovar kurstaki 4A3 ?
-
?
additional information N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence Bacillus thuringiensis serovar kurstaki BGSC 4A3 ?
-
?
N-hexanoyl-(S)-homoserine lactone
-
Bacillus thuringiensis serovar kurstaki N-hexanoyl-(S)-homoserine
-
?
N-hexanoyl-(S)-homoserine lactone
-
Bacillus thuringiensis serovar kurstaki 4A3 N-hexanoyl-(S)-homoserine
-
?
N-hexanoyl-(S)-homoserine lactone
-
Bacillus thuringiensis serovar kurstaki BGSC 4A3 N-hexanoyl-(S)-homoserine
-
?
N-hexanoyl-(S)-homoserine lactone + H2O no activity with N-hexanoyl-(R)-homoserine lactone Bacillus thuringiensis serovar kurstaki N-hexanoyl-(S)-homoserine
-
?
N-hexanoyl-(S)-homoserine lactone + H2O no activity with N-hexanoyl-(R)-homoserine lactone Bacillus thuringiensis serovar kurstaki 4A3 N-hexanoyl-(S)-homoserine
-
?
N-hexanoyl-(S)-homoserine lactone + H2O no activity with N-hexanoyl-(R)-homoserine lactone Bacillus thuringiensis serovar kurstaki BGSC 4A3 N-hexanoyl-(S)-homoserine
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Bacillus thuringiensis serovar kurstaki
? x * 29000, recombinant detagged enzyme, SDS-PAGE Bacillus thuringiensis serovar kurstaki

Synonyms

Synonyms Comment Organism
AHL lactonase
-
Bacillus thuringiensis serovar kurstaki
quorum-quenching lactonase
-
Bacillus thuringiensis serovar kurstaki

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Bacillus thuringiensis serovar kurstaki

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
117
-
N-hexanoyl-(S)-homoserine lactone pH 7.4, 28°C, purified detagged recombinant enzyme Bacillus thuringiensis serovar kurstaki

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Bacillus thuringiensis serovar kurstaki