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Literature summary for 3.1.1.8 extracted from
- Purification of recombinant human butyrylcholinesterase on Hupresin? (2018), J. Chromatogr. B, 1102-1103, 109-115 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of truncated human enzyme in stably transfected CHO-K1 cells, the recombinant enzyme is secreted | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a truncated L530stop, 4 sugars off HuBChE mutant. The deleted glycosylation sites N17/455/481/486 unmask N485 for glycosylation, so that the rHuBChE contains 6 glycans. N485 is not glycated in plasma-derived HuBChE | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| huprine 19 | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | Homo sapiens | - |
choline + butyrate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P06276 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the deleted glycosylation sites N17/455/481/486 unmask N485 for glycosylation, so that the recombinant HuBChE contains 6 glycans. N485 is not glycated in native plasma-derived HuBChE | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular truncated human enzyme from CHO-K1 cell culture medium by Hupresin affinity chromatography, elution is best with 0.1M tetramethylammonium bromide in 20mM TrisCl, pH 7.5, followed by dialysis and ultrafiltration, to homogeneity. Loss of 24% during the dialysis and concentration steps | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood plasma | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 630 | - |
purified recombinant truncated enzyme, pH 7.0, 25°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | - |
Homo sapiens | choline + butyrate | - |
? | |
| butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | native plasma-derived HuBChE tetramer has a nonreducible dimer band at 170 kDa and a monomer band at 85 kDa for 574 amino acids (68419 Da) plus 9 glycans per subunit | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BChE | - |
Homo sapiens |
| butyrylcholinesterase | - |
Homo sapiens |
| HuBChE | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00099 | - |
pH 7.0, 25°C, recombinant enzyme | Homo sapiens | huprine 19 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | construction of Hupresin affinity resin by coupling the huprine (inhibitor) ligand to Sepharose 4B via a 10-atom spacer between the Sepharose beads and the ligand. The spacer extends the ligand out of the Sepharose backbone, making room for the BChE protein to interact with the ligand. Hupresin compared to procainamide affinity gel for purifying BChE, method evaluation, overview. Hupresin is superior to procainamide-resin for purification of BChE, but is not suitable for purifying native acetylcholinesterase, AChE (EC 3.1.1.7), because Hupresin binds AChE so tightly that AChE is not released with buffers, but is desorbed with denaturing solvents such as 50% acetonitrile or 1% trifluoroacetic acid. Procainamide-Sepharose will continue to be useful for purification of AChE | Homo sapiens |
| physiological function | human butyrylcholinesterase (HuBChE) is an efficient bioscavenger of organophosphorus nerve agents | Homo sapiens |
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