Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azinphosmethyl-oxon | - |
Sparus aurata | |
dichlorvos | - |
Sparus aurata | |
additional information | in vitro sensitivity of cholinesterases from gilthead seabream (Sparus aurata) to organophosphate pesticides, overview. Muscle BChE is found to be several orders of magnitude (from 2 to 4) more sensitive than brain and muscle acetylcholinesterase (AChE, EC 3.1.1.7) inhibition by dichlorvos and omethoate. Enzyme BChE does not show excess substrate inhibition | Sparus aurata | |
omethoate | - |
Sparus aurata | |
tetraisopropylpyrophosphoramide | iso-OMPA, enzyme BChE is selectively and completely inhibited | Sparus aurata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Sparus aurata | |
0.16 | - |
butyrylthiocholine | pH 7.4, 25°C, enzyme from muscle | Sparus aurata | |
0.163 | - |
butyrylthiocholine | pH 7.4, 25°C, enzyme from yolk-sac of seabream larvae stage | Sparus aurata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylcholine + H2O | Sparus aurata | - |
choline + butyrate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sparus aurata | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low enzyme level | Sparus aurata | - |
additional information | acetylcholinesterase (AChE, EC 3.1.1.7) is the enzyme present in the brain, whereas in muscle, a typical formof AChE is present along with an atypical form of butyrylcholinesterase (BChE) | Sparus aurata | - |
muscle | - |
Sparus aurata | - |
plasma cell | low enzyme level | Sparus aurata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | enzyme BChE may hydrolyze in some extent the substrate acetylthiocholine, ASCh | Sparus aurata | thiocholine + acetate | - |
? | |
butyrylcholine + H2O | - |
Sparus aurata | choline + butyrate | - |
? | |
butyrylthiocholine + H2O | - |
Sparus aurata | thiocholine + butyrate | - |
? | |
additional information | BChE hydrolyzes a wider range of esters but preferentially S-butyrylthiocholine iodide (BSCh) | Sparus aurata | ? | - |
? | |
propionylthiocholine + H2O | - |
Sparus aurata | thiocholine + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Sparus aurata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sparus aurata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Sparus aurata |