Cloned (Comment) | Organism |
---|---|
C-terminally truncated human enzyme (BChE) is genetically fused to the N-terminal of the Fc portion of wild-type human IgG (Fc(WT)) by overlapping extension PCR, cloned and ligated to the pCMV-MCS expression vector and expressed in CHO-S cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
(-)-cocaine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
0.12 | - |
heroin | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
8.6 | - |
6-monoacetylmorphine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylcholine + H2O | Homo sapiens | - |
choline + butyrate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P06276 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally truncated enzyme BChE from CHO-S cells by affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-cocaine + H2O | - |
Homo sapiens | ecgonine methyl ester + benzoate | - |
? | |
6-monoacetylmorphine + H2O | - |
Homo sapiens | morphine + acetate | - |
? | |
butyrylcholine + H2O | - |
Homo sapiens | choline + butyrate | - |
? | |
heroin + H2O | - |
Homo sapiens | 6-monoacetylmorphine + acetate | - |
? | |
additional information | substrate specificities compared to acetylcholinesterase (EC 3.1.1.7) and cocaine esterase (EC 3.1.1.84) | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Homo sapiens |
butyrylcholinesterase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
6-monoacetylmorphine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
0.068 | - |
(-)-cocaine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
30.67 | - |
heroin | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure homology modelling, molecular dynamics simulations on the structures of enzyme-substrate complexes using the crystal structures of AChE (PDB ID 1B41), and BChE (PDB IDs 2XQF and 1P0P), molecular docking, overview. The positively charged amino-group of the substrates (heroin and 6-monoacetylmorphine) is placed in the choline-binding site near Trp82 in BChE and CocH1 or Trp86 in AChE. The binding models of heroin and 6-monoacetylmorphine in the corresponding enzyme-substrate complexes are optimized by performing the energy minimization | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
6-monoacetylmorphine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
15.19 | - |
(-)-cocaine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
255.56 | - |
heroin | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |