Application | Comment | Organism |
---|---|---|
medicine | the efficient downstream processing scheme and functionality of regulated transgenic enzyme rrBChE confirm its promise as a cost-effective alternative to human BChE for prophylactic and therapeutic use | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant regulated BChE (rrBChE) differs from the wild-type in that it contains plant-type complex N-glycans, including an alpha-1,3 linked core fucose, and a beta-1,2 xylose, and lacking a terminal sialic acid. Despite this difference, rrBChE is demonstrated to be an effective stoichiometric bioscavenger for five different organophosphorous nerve agents in vitro | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclosarin | strongest inhibitor | Oryza sativa | |
huprine X | - |
Oryza sativa | |
O-ethyl-S-2-diisopropylaminoethylmethylphosphonothiolate | VX | Oryza sativa | |
sarin | - |
Oryza sativa | |
soman | - |
Oryza sativa | |
Tabun | - |
Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
butyrylthiocholine | pH 7.4, 25°C, recombinant enzyme | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | site-specific and overall N-glycan structures on recombinant rBChE, glycosylation sites are N47, N241, N256, N341, N481, N455, N485, N481, and N486 | Oryza sativa |
Purification (Comment) | Organism |
---|---|
active recombinant enzyme rrBChE from rice cells to 95% purity by tangential flow ultrafiltration, anion exchange chromatography, and Hupresin affinity chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylthiocholine + H2O | - |
Oryza sativa | thiocholine + butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Oryza sativa |
butyrylcholinesterase | - |
Oryza sativa |
rBChE | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | purified recombinant enzyme, pH 7.4, 30 min, completely stable at up to 20°C, loss of about 20% activity at 40°C and of 75% at 50°C, inactivation at 60°C | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2066.7 | - |
butyrylthiocholine | pH 7.4, 25°C, recombinant enzyme | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the enzyme shows very low (but non-zero) activity at pH 5.0, with a gradual rise in activity to pH 8.0 | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
additional information | structure and activity comparisons of Oryzsa sativa and Homo sapiens enzymes, overview | Oryza sativa |