Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.8 extracted from

  • Corbin, J.M.; Kailemia, M.J.; Cadieux, C.L.; Alkanaimsh, S.; Karuppanan, K.; Rodriguez, R.L.; Lebrilla, C.B.; Cerasoli, D.M.; McDonald, K.A.; Nandi, S.
    Purification, characterization, and N-glycosylation of recombinant butyrylcholinesterase from transgenic rice cell suspension cultures (2018), Biotechnol. Bioeng., 115, 1301-1310 .
    View publication on PubMed

Application

Application Comment Organism
medicine the efficient downstream processing scheme and functionality of regulated transgenic enzyme rrBChE confirm its promise as a cost-effective alternative to human BChE for prophylactic and therapeutic use Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information recombinant regulated BChE (rrBChE) differs from the wild-type in that it contains plant-type complex N-glycans, including an alpha-1,3 linked core fucose, and a beta-1,2 xylose, and lacking a terminal sialic acid. Despite this difference, rrBChE is demonstrated to be an effective stoichiometric bioscavenger for five different organophosphorous nerve agents in vitro Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
cyclosarin strongest inhibitor Oryza sativa
huprine X
-
Oryza sativa
O-ethyl-S-2-diisopropylaminoethylmethylphosphonothiolate VX Oryza sativa
sarin
-
Oryza sativa
soman
-
Oryza sativa
Tabun
-
Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.112
-
butyrylthiocholine pH 7.4, 25°C, recombinant enzyme Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein site-specific and overall N-glycan structures on recombinant rBChE, glycosylation sites are N47, N241, N256, N341, N481, N455, N485, N481, and N486 Oryza sativa

Purification (Commentary)

Purification (Comment) Organism
active recombinant enzyme rrBChE from rice cells to 95% purity by tangential flow ultrafiltration, anion exchange chromatography, and Hupresin affinity chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyrylthiocholine + H2O
-
Oryza sativa thiocholine + butyrate
-
?

Synonyms

Synonyms Comment Organism
BChE
-
Oryza sativa
butyrylcholinesterase
-
Oryza sativa
rBChE
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 purified recombinant enzyme, pH 7.4, 30 min, completely stable at up to 20°C, loss of about 20% activity at 40°C and of 75% at 50°C, inactivation at 60°C Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2066.7
-
butyrylthiocholine pH 7.4, 25°C, recombinant enzyme Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5 8 the enzyme shows very low (but non-zero) activity at pH 5.0, with a gradual rise in activity to pH 8.0 Oryza sativa

General Information

General Information Comment Organism
additional information structure and activity comparisons of Oryzsa sativa and Homo sapiens enzymes, overview Oryza sativa