Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | substrate inhibition with formylthiocholine does not occur | Equus caballus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic isotope effects for the butrylylcholinesterase catalyzed and non-enzymatic hydrolysis of formylthiocholine, overview | Equus caballus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylcholine + H2O | Equus caballus | - |
choline + butyrate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equus caballus | P81908 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acylcholine + H2O = choline + a carboxylate | the enzyme follows a mechanism similar to that of a serine protease in which the activated serine attacks the acyl-substrate to form an acyl-enzyme intermediate. The acyl-enzyme intermediate then reacts directly with a water molecule at the active site. Both the acylation and deacylation reactions occur through a tetrahedral intermediate | Equus caballus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Equus caballus | - |
serum | - |
Equus caballus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | some step (or steps) other than chemical formation of the tetrahedral intermediate are partially rate determining | Equus caballus | thiocholine + acetate | - |
? | |
butyrylcholine + H2O | - |
Equus caballus | choline + butyrate | - |
? | |
butyrylthiocholine + H2O | - |
Equus caballus | thiocholine + butyrate | - |
? | |
formylthiocholine + H2O | via formation of the tetrahedral intermediate, measurement of heavy atom kinetic isotope effects | Equus caballus | thiocholine + formate | - |
? | |
additional information | the observed heavy atom kinetic isotope effects of butyrylcholinesterase-catalyzed hydrolysis of formylthiocholine support a mechanism for both acylation and deacylation where the steps up to and including the formation of the tetrahedral intermediate are at least partially rate determining. The first irreversible step is the product release in acylation | Equus caballus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Equus caballus |
butyrylcholinesterase | - |
Equus caballus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Equus caballus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is thought to be primarily responsible for detoxification reactions in the serum, liver, lungs, and intestinal mucosa | Equus caballus |