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Literature summary for 3.1.1.8 extracted from
- In vitro characterization of cationic copolymer-complexed recombinant human butyrylcholinesterase (2015), Biochem. Pharmacol., 98, 531-539 .
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | effective use of exogenous human BChE as a bioscavenger for organophosphorus toxicants, e.g. paraoxon. Paraoxon elicites near complete inhibition of liver carboxylesterase at 40 nM | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the enzyme in C57BL/6 mice | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | preparation of copolymer-rhBChE complexes (C-BCs) based on one of nine different copolymers, from combinations of three molecular weights (MW) of poly-L-lysine (PLL, high MW, 30-70 kDa, medium MW, 15-30 kDa, low MW, 4-15 kDa) and three grafting ratios of poly(ethylene glycol) (PEG, 2:1, 10:1, 20:1) for complexing of the enzyme as improved bioscavenger for organophosphorus toxicants, method evaluation, overview. Similar sensitivity to in vitro inhibition of BChE activity is noted with the free enzyme and various C-BCs | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethopropazine | - |
Homo sapiens |  |
| paraoxon | similar sensitivity to in vitro inhibition of BChE activity is noted with the free enzyme and various copolymer-rhBChE complexes (C-BCs) | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | Homo sapiens | - |
choline + butyrate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P06276 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from C57BL/6 mice serum by dialysis, anion exchange chromatography, desalting gel filtration, and procainamide affinity chromatography, followed by ultrafiltration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood plasma | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the purified recombinant enzyme is stable during storage at 4°C in 20 mM potassium phosphate, 1 mM EDTA, pH 7.0, containing 0.02% NaN3 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | - |
Homo sapiens | choline + butyrate | - |
? | |
| butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BChE | - |
Homo sapiens |
| butyrylcholinesterase | - |
Homo sapiens |
| hBChE | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at, radiometric method | Homo sapiens |
| 37 | - |
assay at, 5,5'-dithio-bis-(2-nitrobenzoic acid) colorimetric method | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
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