Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetylthiocholine | - |
Homo sapiens | |
butyrylthiocholine | - |
Homo sapiens | |
additional information | enzyme HuBChE shows marked substrate activation with butyrylthiocholine, acetylthiocholine, and propionylthiocholine | Homo sapiens | |
additional information | enzyme PoBChE does not show substrate activation with acetylthiocholine, propionylthiocholine, and butyrylthiocholine | Sus scrofa | |
propionylthiocholine | - |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-huperzine A | - |
Homo sapiens | |
(-)-huperzine A | - |
Sus scrofa | |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | i.e. BW284c51 | Homo sapiens | |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | i.e. BW284c51 | Sus scrofa | |
acetylthiocholine | slight substrate inhibition | Sus scrofa | |
butyrylthiocholine | slight substrate inhibition | Sus scrofa | |
decamethonium | - |
Homo sapiens | |
decamethonium | - |
Sus scrofa | |
diisopropyl fluorophosphate | inhibition, aging, and reactivation kinetics | Homo sapiens | |
diisopropyl fluorophosphate | inhibition, aging, and reactivation kinetics | Sus scrofa | |
edrophonium | - |
Homo sapiens | |
edrophonium | - |
Sus scrofa | |
ethopropazine | - |
Homo sapiens | |
ethopropazine | - |
Sus scrofa | |
additional information | PoBChE is sensitive to BChE-specific inhibitors, but shows only slight substrate inhibition | Sus scrofa | |
phenothiazine | - |
Homo sapiens | |
phenothiazine | - |
Sus scrofa | |
propidium | - |
Homo sapiens | |
propidium | - |
Sus scrofa | |
propionylthiocholine | slight substrate inhibition | Sus scrofa | |
tacrine | - |
Homo sapiens | |
tacrine | - |
Sus scrofa | |
tetra(monoisopropyl)diphosphortetramide | irreversible | Homo sapiens | |
tetra(monoisopropyl)diphosphortetramide | irreversible | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
0.017 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
0.031 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
0.23 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
0.25 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
0.37 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
340000 | - |
native PAGE | Sus scrofa |
342000 | 353000 | analytical ultracentrifugation | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylcholine + H2O | Homo sapiens | - |
choline + butyrate | - |
? | |
butyrylcholine + H2O | Sus scrofa | - |
choline + butyrate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P06276 | - |
- |
Sus scrofa | P32752 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme BChE from defatted milk by procainamide affinity chromatography and ultrafiltration, reduced and denatured PoBChE purified by hupresin affinity chromatography | Sus scrofa |
native enzyme is purified from Cohn fraction IV-4 paste | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Homo sapiens | - |
milk | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | 650 | purified native enzyme from milk, pH 8.0, 22°C | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | - |
Homo sapiens | thiocholine + acetate | - |
? | |
acetylthiocholine + H2O | - |
Sus scrofa | thiocholine + acetate | - |
? | |
butyrylcholine + H2O | - |
Homo sapiens | choline + butyrate | - |
? | |
butyrylcholine + H2O | - |
Sus scrofa | choline + butyrate | - |
? | |
butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
butyrylthiocholine + H2O | - |
Sus scrofa | thiocholine + butyrate | - |
? | |
additional information | PoBChE hydrolyzes acetylthiocholine slightly faster than butyrylthiocholine | Sus scrofa | ? | - |
? | |
propionylthiocholine + H2O | - |
Homo sapiens | thiocholine + propionate | - |
? | |
propionylthiocholine + H2O | - |
Sus scrofa | thiocholine + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 85000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Homo sapiens |
BChE | - |
Sus scrofa |
butyrylcholinesterase | - |
Homo sapiens |
butyrylcholinesterase | - |
Sus scrofa |
HuBChE | - |
Homo sapiens |
PoBChE | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
22 | - |
assay at | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.78 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
5.6 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
38.3 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
50 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
53.3 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000043 | - |
tacrine | pH 8.0, 22°C | Sus scrofa | |
0.000008 | - |
tacrine | pH 8.0, 22°C | Homo sapiens | |
0.00005 | - |
tetra(monoisopropyl)diphosphortetramide | pH 8.0, 22°C | Homo sapiens | |
0.000051 | - |
ethopropazine | pH 8.0, 22°C | Homo sapiens | |
0.00006 | - |
tetra(monoisopropyl)diphosphortetramide | pH 8.0, 22°C | Sus scrofa | |
0.00019 | - |
ethopropazine | pH 8.0, 22°C | Sus scrofa | |
0.0004 | - |
propidium | pH 8.0, 22°C | Homo sapiens | |
0.0008 | - |
decamethonium | pH 8.0, 22°C | Homo sapiens | |
0.0019 | - |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | pH 8.0, 22°C | Homo sapiens | |
0.0093 | - |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | pH 8.0, 22°C | Sus scrofa | |
0.0117 | - |
propidium | pH 8.0, 22°C | Sus scrofa | |
0.0185 | - |
edrophonium | pH 8.0, 22°C | Sus scrofa | |
0.0286 | - |
(-)-huperzine A | pH 8.0, 22°C | Sus scrofa | |
0.0504 | - |
edrophonium | pH 8.0, 22°C | Homo sapiens | |
0.0756 | - |
(-)-huperzine A | pH 8.0, 22°C | Homo sapiens | |
0.0875 | - |
decamethonium | pH 8.0, 22°C | Sus scrofa |
General Information | Comment | Organism |
---|---|---|
additional information | the concentration of active sites in PoBChE is determined by titration with 7-(O,O-diethyl-phosphinyloxy)-1-methylquinolinium methylsulfate (DEPQ) | Sus scrofa |
physiological function | PoBChE hydrolyzes acetylthiocholine slightly faster than butyrylthiocholine, but is sensitive to BChE-specific inhibitors | Sus scrofa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.14 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
20.78 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
1235.5 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
3135.3 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
3333.3 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens |