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Literature summary for 3.1.1.8 extracted from

  • Saxena, A.; Belinskaya, T.; Schopfer, L.M.; Lockridge, O.
    Characterization of butyrylcholinesterase from porcine milk (2018), Arch. Biochem. Biophys., 652, 38-49 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
acetylthiocholine
-
Homo sapiens
butyrylthiocholine
-
Homo sapiens
additional information enzyme HuBChE shows marked substrate activation with butyrylthiocholine, acetylthiocholine, and propionylthiocholine Homo sapiens
additional information enzyme PoBChE does not show substrate activation with acetylthiocholine, propionylthiocholine, and butyrylthiocholine Sus scrofa
propionylthiocholine
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(-)-huperzine A
-
Homo sapiens
(-)-huperzine A
-
Sus scrofa
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide i.e. BW284c51 Homo sapiens
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide i.e. BW284c51 Sus scrofa
acetylthiocholine slight substrate inhibition Sus scrofa
butyrylthiocholine slight substrate inhibition Sus scrofa
decamethonium
-
Homo sapiens
decamethonium
-
Sus scrofa
diisopropyl fluorophosphate inhibition, aging, and reactivation kinetics Homo sapiens
diisopropyl fluorophosphate inhibition, aging, and reactivation kinetics Sus scrofa
edrophonium
-
Homo sapiens
edrophonium
-
Sus scrofa
ethopropazine
-
Homo sapiens
ethopropazine
-
Sus scrofa
additional information PoBChE is sensitive to BChE-specific inhibitors, but shows only slight substrate inhibition Sus scrofa
phenothiazine
-
Homo sapiens
phenothiazine
-
Sus scrofa
propidium
-
Homo sapiens
propidium
-
Sus scrofa
propionylthiocholine slight substrate inhibition Sus scrofa
tacrine
-
Homo sapiens
tacrine
-
Sus scrofa
tetra(monoisopropyl)diphosphortetramide irreversible Homo sapiens
tetra(monoisopropyl)diphosphortetramide irreversible Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
butyrylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
0.017
-
propionylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
0.031
-
acetylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
0.23
-
butyrylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa
0.25
-
propionylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa
0.37
-
acetylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
340000
-
native PAGE Sus scrofa
342000 353000 analytical ultracentrifugation Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butyrylcholine + H2O Homo sapiens
-
choline + butyrate
-
?
butyrylcholine + H2O Sus scrofa
-
choline + butyrate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P06276
-
-
Sus scrofa P32752
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme BChE from defatted milk by procainamide affinity chromatography and ultrafiltration, reduced and denatured PoBChE purified by hupresin affinity chromatography Sus scrofa
native enzyme is purified from Cohn fraction IV-4 paste Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
milk
-
Homo sapiens
-
milk
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
360 650 purified native enzyme from milk, pH 8.0, 22°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine + H2O
-
Homo sapiens thiocholine + acetate
-
?
acetylthiocholine + H2O
-
Sus scrofa thiocholine + acetate
-
?
butyrylcholine + H2O
-
Homo sapiens choline + butyrate
-
?
butyrylcholine + H2O
-
Sus scrofa choline + butyrate
-
?
butyrylthiocholine + H2O
-
Homo sapiens thiocholine + butyrate
-
?
butyrylthiocholine + H2O
-
Sus scrofa thiocholine + butyrate
-
?
additional information PoBChE hydrolyzes acetylthiocholine slightly faster than butyrylthiocholine Sus scrofa ?
-
?
propionylthiocholine + H2O
-
Homo sapiens thiocholine + propionate
-
?
propionylthiocholine + H2O
-
Sus scrofa thiocholine + propionate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 85000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
BChE
-
Homo sapiens
BChE
-
Sus scrofa
butyrylcholinesterase
-
Homo sapiens
butyrylcholinesterase
-
Sus scrofa
HuBChE
-
Homo sapiens
PoBChE
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Homo sapiens
22
-
assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.78
-
butyrylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa
5.6
-
acetylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa
38.3
-
acetylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
50
-
butyrylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
53.3
-
propionylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens
8
-
assay at Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000043
-
tacrine pH 8.0, 22°C Sus scrofa
0.000008
-
tacrine pH 8.0, 22°C Homo sapiens
0.00005
-
tetra(monoisopropyl)diphosphortetramide pH 8.0, 22°C Homo sapiens
0.000051
-
ethopropazine pH 8.0, 22°C Homo sapiens
0.00006
-
tetra(monoisopropyl)diphosphortetramide pH 8.0, 22°C Sus scrofa
0.00019
-
ethopropazine pH 8.0, 22°C Sus scrofa
0.0004
-
propidium pH 8.0, 22°C Homo sapiens
0.0008
-
decamethonium pH 8.0, 22°C Homo sapiens
0.0019
-
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide pH 8.0, 22°C Homo sapiens
0.0093
-
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide pH 8.0, 22°C Sus scrofa
0.0117
-
propidium pH 8.0, 22°C Sus scrofa
0.0185
-
edrophonium pH 8.0, 22°C Sus scrofa
0.0286
-
(-)-huperzine A pH 8.0, 22°C Sus scrofa
0.0504
-
edrophonium pH 8.0, 22°C Homo sapiens
0.0756
-
(-)-huperzine A pH 8.0, 22°C Homo sapiens
0.0875
-
decamethonium pH 8.0, 22°C Sus scrofa

General Information

General Information Comment Organism
additional information the concentration of active sites in PoBChE is determined by titration with 7-(O,O-diethyl-phosphinyloxy)-1-methylquinolinium methylsulfate (DEPQ) Sus scrofa
physiological function PoBChE hydrolyzes acetylthiocholine slightly faster than butyrylthiocholine, but is sensitive to BChE-specific inhibitors Sus scrofa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.14
-
acetylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa
20.78
-
butyrylthiocholine recombinant enzyme, pH 8.0, 22°C Sus scrofa
1235.5
-
acetylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
3135.3
-
propionylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens
3333.3
-
butyrylthiocholine recombinant enzyme, pH 8.0, 22°C Homo sapiens