Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A328F | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
A328G | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
A328I | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
A328W | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
A328Y | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
E197Q | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
L286W | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
V288W | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
Y332A | mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acylcholine + H2O = choline + a carboxylate | mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
butyrylthiocholine | pH 5, wild-type enzyme, absence of excess substrate | Homo sapiens | |
0.05 | - |
butyrylthiocholine | pH 5, A328I mutant, presence of excess substrate | Homo sapiens | |
0.4 | - |
butyrylthiocholine | pH 5, A328W and E197Q mutant, absence of excess substrate | Homo sapiens | |
2 | - |
butyrylthiocholine | pH 5, L286W mutant, absence of excess substrate | Homo sapiens | |
20.5 | - |
butyrylthiocholine | pH 5, A328I mutant, absence of excess substrate | Homo sapiens | |
22.3 | - |
butyrylthiocholine | pH 5, A328G mutant, absence of excess substrate | Homo sapiens | |
41.4 | - |
butyrylthiocholine | pH 5, Y332A mutant, presence of excess substrate | Homo sapiens | |
46.7 | - |
butyrylthiocholine | pH 5, wild-type enzyme, absence of excess substrate | Homo sapiens | |
48.3 | - |
butyrylthiocholine | pH 5, Y332A mutant, absence of excess substrate | Homo sapiens | |
53.3 | - |
butyrylthiocholine | pH 5, A328W and E197Q mutant, absence of excess substrate | Homo sapiens | |
71.7 | - |
butyrylthiocholine | pH 5, L286W mutant, absence of excess substrate | Homo sapiens | |
105 | - |
butyrylthiocholine | pH 5, V288W mutant, absence of excess substrate | Homo sapiens | |
110 | - |
butyrylthiocholine | pH 5, A328F mutant, absence of excess substrate | Homo sapiens | |
290 | - |
butyrylthiocholine | pH 5, L286W mutant, presence of excess substrate | Homo sapiens | |
920 | - |
butyrylthiocholine | pH 5, A328F mutant, presence of excess substrate | Homo sapiens | |
1048 | - |
butyrylthiocholine | pH 5, V288W mutant, presence of excess substrate | Homo sapiens | |
1050 | - |
butyrylthiocholine | pH 5, V288W mutant, presence of excess substrate | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
enzyme retains activity at pH 5 and below under conditions of excess substrate activation | Homo sapiens |