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Literature summary for 3.1.1.8 extracted from

  • Masson, P.; Nachon, F.; Bartels, C.F.; Froment, M.T.; Ribes, F.; Matthews, C.; Lockridge, O.
    High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine (2003), Eur. J. Biochem., 270, 315-324.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
A328F mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
A328G mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
A328I mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
A328W mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
A328Y mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
E197Q mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
L286W mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
V288W mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
Y332A mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an acylcholine + H2O = choline + a carboxylate mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyrylthiocholine + H2O
-
Homo sapiens thiocholine + butyrate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
butyrylthiocholine pH 5, wild-type enzyme, absence of excess substrate Homo sapiens
0.05
-
butyrylthiocholine pH 5, A328I mutant, presence of excess substrate Homo sapiens
0.4
-
butyrylthiocholine pH 5, A328W and E197Q mutant, absence of excess substrate Homo sapiens
2
-
butyrylthiocholine pH 5, L286W mutant, absence of excess substrate Homo sapiens
20.5
-
butyrylthiocholine pH 5, A328I mutant, absence of excess substrate Homo sapiens
22.3
-
butyrylthiocholine pH 5, A328G mutant, absence of excess substrate Homo sapiens
41.4
-
butyrylthiocholine pH 5, Y332A mutant, presence of excess substrate Homo sapiens
46.7
-
butyrylthiocholine pH 5, wild-type enzyme, absence of excess substrate Homo sapiens
48.3
-
butyrylthiocholine pH 5, Y332A mutant, absence of excess substrate Homo sapiens
53.3
-
butyrylthiocholine pH 5, A328W and E197Q mutant, absence of excess substrate Homo sapiens
71.7
-
butyrylthiocholine pH 5, L286W mutant, absence of excess substrate Homo sapiens
105
-
butyrylthiocholine pH 5, V288W mutant, absence of excess substrate Homo sapiens
110
-
butyrylthiocholine pH 5, A328F mutant, absence of excess substrate Homo sapiens
290
-
butyrylthiocholine pH 5, L286W mutant, presence of excess substrate Homo sapiens
920
-
butyrylthiocholine pH 5, A328F mutant, presence of excess substrate Homo sapiens
1048
-
butyrylthiocholine pH 5, V288W mutant, presence of excess substrate Homo sapiens
1050
-
butyrylthiocholine pH 5, V288W mutant, presence of excess substrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5
-
enzyme retains activity at pH 5 and below under conditions of excess substrate activation Homo sapiens