Literature summary for 3.1.1.79 extracted from

Huang, J.; Huo, Y.; Ji, R.; Kuang, S.; Ji, C.; Xu, X.; Li, J.
Structural insights of a hormone sensitive lipase homologue Est22 (2016), Sci. Rep., 6, 28550 .

Activating Compound

Activating Compound	Comment	Organism
Triton X-100	1%, 130% of initial activity	uncultured bacterium
Tween 20	1%, 200% of initial activity	uncultured bacterium
Tween 80	1%, 160% of initial activity	uncultured bacterium

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of wild type and mutated Est22 with its product 4-nitrophenol, to 1.4-2.43 A. Est22 exhibits an alpha/beta-hydrolase fold consisting of a catalytic domain and a substrate-recognizing cap domain. Residues Ser188, Asp287, and His317 comprise the catalytic triad in the catalytic domain. 4-Nitrophenol occupies the substrate binding pocket and forms hydrogen bonds with adjacent residues Gly108, Gly109, and Gly189	uncultured bacterium

Crystallization (Comment)

Organism

structures of wild type and mutated Est22 with its product 4-nitrophenol, to 1.4-2.43 A. Est22 exhibits an alpha/beta-hydrolase fold consisting of a catalytic domain and a substrate-recognizing cap domain. Residues Ser188, Asp287, and His317 comprise the catalytic triad in the catalytic domain. 4-Nitrophenol occupies the substrate binding pocket and forms hydrogen bonds with adjacent residues Gly108, Gly109, and Gly189

uncultured bacterium

Protein Variants

Protein Variants	Comment	Organism
D287A	active site mutant, significant decrease in activity, mutant forms oligomers instead of dimers	uncultured bacterium
H317A	active site mutant, significant decrease in activity, mutant forms oligomers instead of dimers	uncultured bacterium
S188A	active site mutant, significant decrease in activity	uncultured bacterium

Inhibitors

Inhibitors	Comment	Organism
Ba2+	10 mM, 40% loss of activity	uncultured bacterium
Cu2+	10 mM, complete loss of activity	uncultured bacterium
Ni2+	10 mM, 50% loss of activity	uncultured bacterium
SDS	1%, complete loss of activity	uncultured bacterium
Zn2+	10 mM, complete loss of activity	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.4899	-	4-nitrophenyl acetate	pH 7.5, 40°C	uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
79100	-	gel filtration, multi-angle light scattering	uncultured bacterium

Organic Solvent Stability

Organic Solvent	Comment	Organism
acetonitrile	15%, complete loss of activity	uncultured bacterium
dimethylformamide	15%, 95% loss of activity	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	H6BDX1	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2065	-	substrate 4-nitrophenyl acetate, pH 7.5, 40°C	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	uncultured bacterium	4-nitrophenol + acetate	-	?
additional information	Est22 hydrolyzes 4-nitrophenyl esters up to C8, while the enzymatic activity decreases significantly with increasing chain length	uncultured bacterium	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 238900, calculated from sequence including His-tag	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
Est22	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
35	-	95% of maximum activity	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3160	-	4-nitrophenyl acetate	pH 7.5, 40°C	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	uncultured bacterium