Literature summary for 3.1.1.79 extracted from

Krintel, C.; Osmark, P.; Larsen, M.R.; Resjoe, S.; Logan, D.T.; Holm, C.
Ser649 and Ser650 are the major determinants of protein kinase A-mediated activation of human hormone-sensitive lipase against lipid substrates (2008), PLoS ONE, 3, e3756.

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Cloned(Commentary)

Cloned (Comment)	Organism
five C-terminally His-tagged human HSL variants are successfully cloned and expressed in Sf9 insect cells using the baculovirus/insect cell expression system	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S552A/S554A	variant AVA-SS, no effect on the activity	Homo sapiens
S552E/S554E	variant AVA-AA, decreasing activity of human HSL against trioleoylglycerol, 1,2-dioleoyl-sn-glycerol and cholesteryl oleate substrates by 67%, 63% and 60%, respectively. The activity against p-nitrophenylbutyrate also decreases, but only by 30% compared to wild type HSL	Homo sapiens
S554A	variant SVA-SS, resulted in a slight decrease in lipolytic activity	Homo sapiens
S554E	variant SVE-SS, resulted in similar to wild type activity	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q05469	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange chromatography followed by nickel affinity chromatography and dialysis	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.2	-	specific activity of the purified wild type enzyme against trioleoylglycerol	Homo sapiens
2.3	-	specific activity of the purified wild type enzyme against cholesteryl oleate	Homo sapiens
23	-	specific activity of the purified wild type enzyme against 1,2-dioleoyl-sn-glycerol	Homo sapiens
50	-	specific activity of the purified wild type enzyme against p-nitrophenylbutyrate	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-dioleoyl-sn-glycerol + H2O	-	Homo sapiens	?	-	?
cholesteryl oleate + H2O	-	Homo sapiens	?	-	?
p-nitrophenyl butyrate + H2O	-	Homo sapiens	p-nitrophenol + butanoate	-	?
trioleoylglycerol + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
hormone-sensitive lipase	-	Homo sapiens
HSL	-	Homo sapiens

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Release 2023.1 (January 2023)