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Literature summary for 3.1.1.79 extracted from
- SSoNDELTA and SSoNDELTAlong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus? (2007), Archaea, 2, 109-115.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
p-nitrophenyl hexanoate | 50°C, SsoNDELTAlong | Saccharolobus solfataricus |  |
| 0.054 | - |
p-nitrophenyl hexanoate | 50°C, SsoNDELTA | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97VU2 | SSO2517 can be expressed as SsoNDELTA or SsoNDELTAlong (longer version of SsoNDELTA with an extended N-terminus) | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl hexanoate + H2O | SsoNDELTAlong, is 15fold more active with the substrate p-nitrophenyl hexanoate than SsoNDELTA | Saccharolobus solfataricus | p-nitrophenol + hexanoate | - |
? | |
| p-nitrophenyl-dodecanoate + H2O | SsoNDELTA shows 4.4fold lower activity than SsoNDELTAlong with p-nitrophenyl-dodecanoate as substrate | Saccharolobus solfataricus | p-nitrophenol + dodecanoate | - |
? | |
| tributyrylglycerol + H2O | SsoNDELTA shows 6old lower activity than SsoNDELTAlong with glyceryl tributyrate as substrate | Saccharolobus solfataricus | ? | - |
? | |
| tridecanoylglycerol + H2O | SsoNDELTA shows 4.7fold higher activity than SsoNDELTAlong with glyceryl tridecanoate as substrate | Saccharolobus solfataricus | ? | - |
? | |
| trihexanoylglycerol + H2O | SsoNDELTA shows 4fold lower activity than SsoNDELTAlong with glyceryl trihexanoate as substrate | Saccharolobus solfataricus | ? | - |
? | |
| trioctanoylglycerol + H2O | SsoNDELTA shows 1.7fold lower activity than SsoNDELTAlong with glyceryl trioctanoate as substrate | Saccharolobus solfataricus | ? | - |
? | |
| trioleoylglycerol + H2O | SsoNDELTA shows 10fold higher activity than SsoNDELTAlong with glyceryl trioleate as substrate | Saccharolobus solfataricus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hormone-sensitive lipase | - |
Saccharolobus solfataricus |
| SSO2517 | - |
Saccharolobus solfataricus |
| SsoNDELTA | - |
Saccharolobus solfataricus |
| SsoNDELTAlong | longer version of SsoNDELTA the protein with an extended N-terminus | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
substrate: p-nitrophenyl hexanoate, SsoNDELTAlong | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | 70°C: substrate: about 75% of maximal activity, 90°C: about 90% of maximal activity, p-nitrophenyl hexanoate, SsoNDELTAlong | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
p-nitrophenyl hexanoate | 50°C, SsoNDELTA | Saccharolobus solfataricus | |
| 34.5 | - |
p-nitrophenyl hexanoate | 50°C, SsoNDELTAlong | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
SsoNDELTAlong, substrate: p-nitrophenyl hexanoate | Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | pH 4.5: 80% of maximal activity, pH 7.5: about 65% of maximal activity, substrate: p-nitrophenyl hexanoate, SsoNDELTAlong | Saccharolobus solfataricus |
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