Any feedback?
Literature summary for 3.1.1.79 extracted from
- Mutational analysis of the "regulatory module" of hormone-sensitive lipase (2005), Biochemistry, 44, 1953-1959.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA644-658 | reduced basal hydrolytic activity, no activation by cyclic AMP-dependent protein kinase | Rattus norvegicus |
| DELTA657-664 | reduced basal hydrolytic activity, no activation by cyclic AMP-dependent protein kinase | Rattus norvegicus |
| DELTA666-683 | very low basal and stimulated hydrolytic activity. Vmax with cholesteryl ester is 29fold lower than wild-type value. Vmax with p-nitrophenylbutyrate is 8fold lower than wild-type value | Rattus norvegicus |
| F650C | reduced activity against lipid-soluble but not water-soluble substrates | Rattus norvegicus |
| F654D | reduced activity against lipid-soluble but not water-soluble substrates | Rattus norvegicus |
| P651A | reduced activity against lipid-soluble but not water-soluble substrates | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
cholesteryl oleate | wild-type enzyme | Rattus norvegicus |  |
| 0.021 | - |
cholesteryl oleate | DELTA666-683 | Rattus norvegicus | |
| 0.024 | - |
cholesteryl oleate | DELTA644-658 | Rattus norvegicus | |
| 0.025 | - |
cholesteryl oleate | DELTA657-664 | Rattus norvegicus | |
| 0.268 | - |
4-nitrophenyl oleate | DELTA666-683 | Rattus norvegicus | |
| 0.389 | - |
4-nitrophenyl oleate | DELTA644-658 | Rattus norvegicus | |
| 0.427 | - |
4-nitrophenyl oleate | DELTA657-664 | Rattus norvegicus | |
| 0.462 | - |
4-nitrophenyl oleate | wild-type enzyme | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | rate-limiting enzyme in lipolysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
recombinant | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl oleate + H2O | - |
Rattus norvegicus | 4-nitrophenol + oleate | - |
? | |
| cholesteryl oleate + H2O | - |
Rattus norvegicus | cholesterol + oleic acid | - |
? | |
| additional information | rate-limiting enzyme in lipolysis | Rattus norvegicus | ? | - |
? | |
| p-nitrophenyl butyrate + H2O | - |
Rattus norvegicus | p-nitrophenol + butanoate | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
