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Literature summary for 3.1.1.79 extracted from

  • Cordle, S.R.; Colbran, R.J.; Yeaman, S.J.
    Hormone-sensitive lipase from bovine adipose tissue (1986), Biochim. Biophys. Acta, 887, 51-57.
    View publication on PubMed
Search for more literature for 3.1.1.79

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate complete inhibition Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
pH 5.0, DE-52, hydroxylapatite, Phenyl-Sepharose, heparin-Sepharose Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue perirenal Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.45
-
 hydrolysis of cholesteryl oleate Bos taurus
30
-
 hydrolysis of 1-oleoyl-2-O-oleylglycerol Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 500 mM phosphate buffer, pH 7.0, 50% glycerol, 100 mM benzamidine, 1 mM dithiothreitol, 0.2% C13E12, 5 mg/ml leupeptin and 1 mg/ml pepstatin, at least 2 months, no loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dioleoyl-sn-glycerol + H2O
-
 Bos taurus 2-oleylglycerol + oleate
-
 ?
cholesteryl oleate + H2O
-
 Bos taurus cholesterol + oleate
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE Bos taurus