BRENDA - Enzyme Database
show all sequences of 3.1.1.77

Identification of acyloxyacyl hydrolase, a lipopolysaccharide-detoxifying enzyme, in the murine urinary tract

feulner, J.A.; Lu, M.; Shelton, J.M.; Zhang, M.; Richardson, J.A.; Munford, R.S.; Infect. Immun. 72, 3171-3178 (2004)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
Mus musculus
deacylation greatly reduces the ability of lipopolysaccharide to stimulate cells via CD14-MD-2-Toll-like receptor 4. Cortical tubule cells may produce and secrete the enzyme to limit inflammatory responses to gram-negative bacteria throughout the urinary tract
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
O35298
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
proximal tubule cells secrete the pro-enzyme, which can be taken up by bladder cells and processed to the heterodimeric, more enzymatically active, mature enzyme form
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
gut
low activity
Mus musculus
-
liver
low activity
Mus musculus
-
renal cortex
renal corticular tubule cells produce AOAH and secrete it into urine, where it can deacylate lipopolysaccharides
Mus musculus
-
spleen
low activity
Mus musculus
-
urinary tract
renal corticular tubule cells produce AOAH and secrete it into urine, where it can deacylate lipopolysaccharides
Mus musculus
-
urine
renal corticular tubule cells produce AOAH and secrete it into urine, where it can deacylate lipopolysaccharides
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
deacylation greatly reduces the ability of lipopolysaccharide to stimulate cells via CD14-MD-2-Toll-like receptor 4. Cortical tubule cells may produce and secrete the enzyme to limit inflammatory responses to gram-negative bacteria throughout the urinary tract
665166
Mus musculus
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin
Salmonella enterica serovar typhimurium lipopolysaccharide is used as substrate
665166
Mus musculus
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
Mus musculus
deacylation greatly reduces the ability of lipopolysaccharide to stimulate cells via CD14-MD-2-Toll-like receptor 4. Cortical tubule cells may produce and secrete the enzyme to limit inflammatory responses to gram-negative bacteria throughout the urinary tract
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
proximal tubule cells secrete the pro-enzyme, which can be taken up by bladder cells and processed to the heterodimeric, more enzymatically active, mature enzyme form
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
gut
low activity
Mus musculus
-
liver
low activity
Mus musculus
-
renal cortex
renal corticular tubule cells produce AOAH and secrete it into urine, where it can deacylate lipopolysaccharides
Mus musculus
-
spleen
low activity
Mus musculus
-
urinary tract
renal corticular tubule cells produce AOAH and secrete it into urine, where it can deacylate lipopolysaccharides
Mus musculus
-
urine
renal corticular tubule cells produce AOAH and secrete it into urine, where it can deacylate lipopolysaccharides
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(acyloxy)acyl group of bacterial toxin
deacylation greatly reduces the ability of lipopolysaccharide to stimulate cells via CD14-MD-2-Toll-like receptor 4. Cortical tubule cells may produce and secrete the enzyme to limit inflammatory responses to gram-negative bacteria throughout the urinary tract
665166
Mus musculus
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
3-(acyloxy)acyl group of bacterial toxin
Salmonella enterica serovar typhimurium lipopolysaccharide is used as substrate
665166
Mus musculus
3-hydroxyacyl group of bacterial toxin + a fatty acid
-
-
-
?
Other publictions for EC 3.1.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749541
Yang
Acyloxyacyl hydrolase modulat ...
Mus musculus
Am. J. Physiol. Regul. Integr. Comp. Physiol.
314
R353-R365
2018
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1
1
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751999
Zou
Acyloxyacyl hydrolase promote ...
Mus musculus
PLoS Pathog.
13
e1006436
2017
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1
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1
1
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730832
Janelsins
Altered inactivation of commen ...
Mus musculus
Proc. Natl. Acad. Sci. USA
111
373-378
2014
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5
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1
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2
2
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715145
Shao
Prolonged hepatomegaly in mice ...
Mus musculus
Hepatology
54
1051-1062
2011
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6
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1
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2
2
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716472
Klaffenbach
Contribution of different plac ...
Homo sapiens
Placenta
32
830-837
2011
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2
1
1
2
-
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704964
Ojogun
Overproduction of acyloxyacyl ...
Mus musculus
J. Infect. Dis.
200
1685-1693
2009
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1
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5
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1
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1
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691683
Lu
Host inactivation of bacterial ...
Mus musculus
Cell host microbe
4
293-302
2008
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1
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4
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680766
Shao
A host lipase detoxifies bacte ...
Mus musculus
J. Biol. Chem.
282
13726-13735
2007
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1
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680889
Gioannini
Endotoxin-binding proteins mod ...
Homo sapiens
J. Biol. Chem.
282
7877-7884
2007
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682968
Mehrzad
Bovine blood neutrophil acylox ...
Bos taurus
Vet. Res.
38
655-668
2007
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1
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3
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1
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680332
Barnes
Polymorphisms in the novel gen ...
Homo sapiens
J. Allergy Clin. Immunol.
118
70-77
2006
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665166
feulner
Identification of acyloxyacyl ...
Mus musculus
Infect. Immun.
72
3171-3178
2004
-
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1
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3
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1
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7
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2
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1
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1
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7
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2
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665875
Lu
Stimulus-dependent deacylation ...
Mus musculus
J. EXp. Med.
197
1745-1754
2003
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246554
Staab
-
Distinctive structural feature ...
Homo sapiens, Mus musculus, Oryctolagus cuniculus
J. Endotoxin Res.
5
205-208
1999
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3
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3
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3
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3
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3
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6
3
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3
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3
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3
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3
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6
3
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246553
Cody
-
Effect of inflammatory and ant ...
Mus musculus
J. Endotoxin Res.
4
371-379
1997
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1
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1
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246552
Staab
A saposin-like domain influenc ...
Homo sapiens
J. Biol. Chem.
269
23736-23742
1994
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1
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1
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2
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1
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246547
Munford
Acyloxyacyl hydrolase, a leuko ...
Homo sapiens
J. Biol. Chem.
267
10116-10121
1992
2
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3
2
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1
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8
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246551
Munford
Eukaryotic lipopolysaccharide ...
Homo sapiens
Methods Enzymol.
209
485-492
1992
1
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1
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246546
Hagen
Expression and characterizatio ...
Homo sapiens
Biochemistry
30
8415-8423
1991
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1
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246550
Mc Dermott
-
Intracellular and extracellula ...
Bos taurus, Homo sapiens
Infect. Immun.
59
487-485
1991
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4
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246545
Erwin
Deacylation of structurally di ...
Homo sapiens
J. Biol. Chem.
265
16444-16449
1990
1
1
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3
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8
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2
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1
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2
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246549
Munford
Purification of acyloxyacyl hy ...
Homo sapiens
J. Biol. Chem.
264
15613-15619
1989
1
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1
1
1
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4
1
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3
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1
1
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1
2
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1
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1
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1
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1
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4
1
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1
1
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2
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2
1
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1
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246548
Munford
Detoxification of bacterial li ...
Homo sapiens
Science
234
203-205
1986
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