Application | Comment | Organism |
---|---|---|
synthesis | construcution of a biosynthetic pathway for the production of (R)-3-hydroxyalkanoates through in vivo depolymerization of poly(3-hydroxyalkanoates) in an Escherichia coli fadA mutant WA101 by introducing the Pseudomonas sp. 61-3 polyhydroxyalkanoate synthase gene (phaC2) and the Pseudomonas aeruginosa intracellular polyhydroxyalkanoate depolymerase gene (phaZ). Upon culture in Luria-Bertani (LB) medium containing 2 g/l of sodium decanoate, 3-hydroxyalkanoats can be produced to the concentration of 0.49 g/l. The mole fractions are 7.5 mol% of 3-hydroxybutanoate, 31.6 mol% of 3-hydroxyhexanoate, 30 mol% of 3-hydroxyoctanoate, 29.4 mol% of 3-hydroxydecanoate, and 1.5 mol% of 3-hydroxydodecanoate | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
PhaZ | - |
Pseudomonas aeruginosa |