Cloned (Comment) | Organism |
---|---|
recombinant expression of the extracellular enzyme in different hosts, e.g. Escherichia coli strains MC4100 (pUC18) and MC4100 (pLJ1) , and Pseudomonas putida strains KT2442, KT42FadB (pIZ1016), or KT42FadB(pIZPZ), method optimization and evaluation, the highest enzyme activity is obtained using Pseudomonas putida strain KT2442 fadB mutant | Pseudomonas fluorescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas fluorescens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens GK13 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant enzyme degrades previously biosynthesized polyhydroxyalkanotes to obtain 3-hydroxyalkanoic acids, it is also active on functionalized medium-chain-length-polyhydroxyalkanotes, i.e. PHACOS, harboring thioester groups in the side chai | Pseudomonas fluorescens | ? | - |
? | |
additional information | the recombinant enzyme degrades previously biosynthesized polyhydroxyalkanotes to obtain 3-hydroxyalkanoic acids, it is also active on functionalized medium-chain-length-polyhydroxyalkanotes, i.e. PHACOS, harboring thioester groups in the side chai | Pseudomonas fluorescens GK13 | ? | - |
? | |
polyhydroxyoctanoate-co-hexanoate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
polyhydroxyoctanoate-co-hexanoate + H2O | - |
Pseudomonas fluorescens GK13 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhaZGK13 | - |
Pseudomonas fluorescens |
polyhydroxyalkanoate depolymerase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas fluorescens |
General Information | Comment | Organism |
---|---|---|
additional information | hydrolyzed functionalized mcl-polyhydroxyalkanotes PHACOS show greater potential to inhibit Staphylococcus aureus growth when compared to that of degradation products of non functionalized polyhydroxyoctanoate-co-hexanoate P(HO-co-HH) | Pseudomonas fluorescens |
physiological function | influence of an active beta-oxidationpathway on the extracellular degradation of polyhydroxyalkanoates, overview | Pseudomonas fluorescens |