Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Pseudomonas putida | |
mercaptoethanol | - |
Pseudomonas putida | |
phenylmethylsulfonyl fluoride | - |
Pseudomonas putida | |
SDS | - |
Pseudomonas putida | |
sodium cholate | - |
Pseudomonas putida | |
Triton X-100 | - |
Pseudomonas putida | |
Tween-20 | - |
Pseudomonas putida | |
Tween-80 | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas putida | 5622 | - |
PHA granule | - |
Pseudomonas putida | 70088 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | for optimal activity, it is important to maintain a high ionic strength (300mM NaCl) in the reaction mixture | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q5Y152 | - |
- |
Pseudomonas putida KT 2442 | Q5Y152 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,3-tributyryl-sn-glycerol + H2O | - |
Pseudomonas putida | ? | - |
? | |
1,2,3-tributyryl-sn-glycerol + H2O | - |
Pseudomonas putida KT 2442 | ? | - |
? | |
additional information | hydrolyzes specifically mcl-PHAs containing aliphatic and aromatic monomers, e.g. P(HO-co-HX)n (HO: 87%, HX: 13%), P(HO-co-HX)s (HO: 87%, HX: 13%), PHPhh, PHPhO, P(HN-co-HP) (HN: 55%, HP: 45%) | Pseudomonas putida | ? | - |
? | |
additional information | hydrolyzes specifically mcl-PHAs containing aliphatic and aromatic monomers, e.g. P(HO-co-HX)n (HO: 87%, HX: 13%), P(HO-co-HX)s (HO: 87%, HX: 13%), PHPhh, PHPhO, P(HN-co-HP) (HN: 55%, HP: 45%) | Pseudomonas putida KT 2442 | ? | - |
? | |
poly(3-hydroxyphenylheptanoate) + H2O | - |
Pseudomonas putida | ? | - |
? | |
poly(3-hydroxyphenylheptanoate) + H2O | - |
Pseudomonas putida KT 2442 | ? | - |
? | |
poly(3-hydroxyphenyloctanoate) + H2O | - |
Pseudomonas putida | ? | - |
? | |
poly(3-hydroxyphenyloctanoate) + H2O | - |
Pseudomonas putida KT 2442 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
medium chain length polyhydroxyalkanoate depolymerase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
very unstable above | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | pH 7.0: about 25% of maximal activity, pH 10.5: about 50% of maximal activity | Pseudomonas putida |