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Literature summary for 3.1.1.76 extracted from

  • Jiang, Y.; Ye, J.; Wu, H.; Zhang, H.
    Cloning and expression of the polyhydroxyalkanoate depolymerase gene from Pseudomonas putida, and characterization of the gene product (2004), Biotechnol. Lett., 26, 1585-1588.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene phaZ, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31283
-
 x * 31283, sequence calculation Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q6PLI2 gene phaZ
-
Pseudomonas putida H14 Q6PLI2 gene phaZ
-

Renatured (Commentary)

Renatured (Comment) Organism
denaturing of the enzyme by Triton buffer, refolding of the enzyme from inclusion bodies after recombinant expression in Escherichia coli in Tris-HCl, pH 8.0, containing GSSG and GSH at 25°C Pseudomonas putida

Subunits

Subunits Comment Organism
? x * 31283, sequence calculation Pseudomonas putida
More the primary sequence contains a conserved lipase box-lke sequence G-X-S102-X-G Pseudomonas putida

Synonyms

Synonyms Comment Organism
PHA depolymerase
-
 Pseudomonas putida
polyhydroxyalkanoate depolymerase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 maximal activity at 40°C, 50% activity of maximal activity at 10°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida