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Literature summary for 3.1.1.76 extracted from
- Characterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Pseudomonas alcaligenes LB19 (2002), Biomacromolecules, 3, 291-296.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by DTT | Pseudomonas alcaligenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Pseudomonas alcaligenes |  |
| additional information | no inhibition by DTT | Pseudomonas alcaligenes | |
| PMSF | - |
Pseudomonas alcaligenes | |
| Triton X-100 | at 0.05% | Pseudomonas alcaligenes | |
| Tween 80 | at 0.1% | Pseudomonas alcaligenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas alcaligenes | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27600 | - |
gel filtration | Pseudomonas alcaligenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas alcaligenes | - |
- |
- |
| Pseudomonas alcaligenes LB19 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by hydrophobic interaction chromatography and gel filtration | Pseudomonas alcaligenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows no activity with poly(3-hydroxybutyrate), polycaprolactone, and poly(L-lactide) | Pseudomonas alcaligenes | ? | - |
? | |
| additional information | the enzyme shows no activity with poly(3-hydroxybutyrate), polycaprolactone, and poly(L-lactide) | Pseudomonas alcaligenes LB19 | ? | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | several bacterial aliphatic and aromatic medium-chain length poly(3-hydroxyalkanoates), aliphatic substrates are preferred | Pseudomonas alcaligenes | oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate | - |
? | |
| poly(3-hydroxyalkanoate) + H2O | several bacterial aliphatic and aromatic medium-chain length poly(3-hydroxyalkanoates), aliphatic substrates are preferred | Pseudomonas alcaligenes LB19 | oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCL-PHA depolymerase | - |
Pseudomonas alcaligenes |
| medium-chain-length poly(3-hydroxyalkanoate) depolymerase | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas alcaligenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas alcaligenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas alcaligenes | - |
- |
5.7 |
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