Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete inhibition | Schlegelella thermodepolymerans | |
4-hydroxymercuribenzoate | - |
Pseudomonas indica | |
4-hydroxymercuribenzoate | complete inhibition | Schlegelella thermodepolymerans | |
diisopropylfluorophosphate | complete inhibition | Schlegelella thermodepolymerans | |
dithiothreitol | complete inhibition | Schlegelella thermodepolymerans | |
DTNB | complete inhibition | Schlegelella thermodepolymerans | |
EDTA | - |
Pseudomonas indica | |
EDTA | complete inhibition | Schlegelella thermodepolymerans | |
ethanol | strong inhibition at 10% v/v | Schlegelella thermodepolymerans | |
iodoacetate | - |
Pseudomonas indica | |
iodoacetate | complete inhibition | Schlegelella thermodepolymerans | |
KCl | - |
Schlegelella thermodepolymerans | |
additional information | no inhibition by DTT | Pseudomonas indica | |
n-Propanol | strong inhibition at 10% v/v | Schlegelella thermodepolymerans | |
NaCl | - |
Schlegelella thermodepolymerans | |
PMSF | 40% inhibition 1.0-10 mM | Schlegelella thermodepolymerans | |
Sodium azide | - |
Pseudomonas indica | |
Sodium azide | complete inhibition | Schlegelella thermodepolymerans | |
Triton X-100 | strong inhibition | Pseudomonas indica | |
Triton X-100 | strong inhibition 0.2-1.0 mM | Schlegelella thermodepolymerans | |
Tween 20 | 66% inhibition 2 mM | Schlegelella thermodepolymerans | |
Tween 80 | strong inhibition | Pseudomonas indica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Schlegelella thermodepolymerans | - |
- |
extracellular | - |
Pseudomonas indica | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Schlegelella thermodepolymerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas indica | - |
- |
- |
Pseudomonas indica K2 / DSM 16298 | - |
- |
- |
Schlegelella thermodepolymerans | - |
strain DSM 15344 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by ultrafiltration, affinity-based separation, and gel filtration | Pseudomonas indica |
to homogeneity by ultrafiltration, heat tretament at 70°C for 30 min, and affinity-based separation | Schlegelella thermodepolymerans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the bacterium can grow on 3-mercaptopropionate or poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source | Schlegelella thermodepolymerans | - |
additional information | the bacterium can grow on poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source | Pseudomonas indica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
purified enzyme | Schlegelella thermodepolymerans |
26 | - |
purified enzyme | Pseudomonas indica |
Storage Stability | Organism |
---|---|
-20°C to 70°C, purified enzyme, stable for more than 6 months | Pseudomonas indica |
-20°C, purified enzyme, 1 year, 70% remaining activity | Schlegelella thermodepolymerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate | Schlegelella thermodepolymerans | ? | - |
? | |
additional information | substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) | Pseudomonas indica | ? | - |
? | |
additional information | substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) | Pseudomonas indica K2 / DSM 16298 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-mercaptopropionate) + H2O | co-polymeric substrate, the activity decreases with increasing amount of 3-mercaptopropionate | Schlegelella thermodepolymerans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Schlegelella thermodepolymerans |
Synonyms | Comment | Organism |
---|---|---|
PHA depolymerase | - |
Schlegelella thermodepolymerans |
PHA depolymerase | - |
Pseudomonas indica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
optimal bacterial growth at 30°C | Pseudomonas indica |
additional information | - |
optimal bacterial growth at 55°C | Schlegelella thermodepolymerans |
35 | - |
- |
Pseudomonas indica |
75 | 90 | - |
Schlegelella thermodepolymerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | - |
Pseudomonas indica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
over 90% remaining activity after 24 h | Schlegelella thermodepolymerans |
70 | - |
69% remaining activity after 24 h | Schlegelella thermodepolymerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Schlegelella thermodepolymerans |
8 | - |
assay at | Pseudomonas indica |