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Literature summary for 3.1.1.76 extracted from

  • Elbanna, K.; Lütke-Eversloh, T.; Jendrossek, D.; Luftmann, H.; Steinbüchel, A.
    Studies on the biodegradability of polythioester copolymers and homopolymers by polyhydroxyalkanoate (PHA)-degrading bacteria and PHA depolymerase (2004), Arch. Microbiol., 182, 212-225.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol complete inhibition Schlegelella thermodepolymerans
4-hydroxymercuribenzoate
-
Pseudomonas indica
4-hydroxymercuribenzoate complete inhibition Schlegelella thermodepolymerans
diisopropylfluorophosphate complete inhibition Schlegelella thermodepolymerans
dithiothreitol complete inhibition Schlegelella thermodepolymerans
DTNB complete inhibition Schlegelella thermodepolymerans
EDTA
-
Pseudomonas indica
EDTA complete inhibition Schlegelella thermodepolymerans
ethanol strong inhibition at 10% v/v Schlegelella thermodepolymerans
iodoacetate
-
Pseudomonas indica
iodoacetate complete inhibition Schlegelella thermodepolymerans
KCl
-
Schlegelella thermodepolymerans
additional information no inhibition by DTT Pseudomonas indica
n-Propanol strong inhibition at 10% v/v Schlegelella thermodepolymerans
NaCl
-
Schlegelella thermodepolymerans
PMSF 40% inhibition 1.0-10 mM Schlegelella thermodepolymerans
Sodium azide
-
Pseudomonas indica
Sodium azide complete inhibition Schlegelella thermodepolymerans
Triton X-100 strong inhibition Pseudomonas indica
Triton X-100 strong inhibition 0.2-1.0 mM Schlegelella thermodepolymerans
Tween 20 66% inhibition 2 mM Schlegelella thermodepolymerans
Tween 80 strong inhibition Pseudomonas indica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Schlegelella thermodepolymerans
-
-
extracellular
-
Pseudomonas indica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, SDS-PAGE Schlegelella thermodepolymerans

Organism

Organism UniProt Comment Textmining
Pseudomonas indica
-
-
-
Pseudomonas indica K2 / DSM 16298
-
-
-
Schlegelella thermodepolymerans
-
strain DSM 15344
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by ultrafiltration, affinity-based separation, and gel filtration Pseudomonas indica
to homogeneity by ultrafiltration, heat tretament at 70°C for 30 min, and affinity-based separation Schlegelella thermodepolymerans

Source Tissue

Source Tissue Comment Organism Textmining
additional information the bacterium can grow on 3-mercaptopropionate or poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source Schlegelella thermodepolymerans
-
additional information the bacterium can grow on poly(3-hydroxybutyrate-co-3-mercaptopropionate) as sole carbon source Pseudomonas indica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
purified enzyme Schlegelella thermodepolymerans
26
-
purified enzyme Pseudomonas indica

Storage Stability

Storage Stability Organism
-20°C to 70°C, purified enzyme, stable for more than 6 months Pseudomonas indica
-20°C, purified enzyme, 1 year, 70% remaining activity Schlegelella thermodepolymerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate Schlegelella thermodepolymerans ?
-
?
additional information substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) Pseudomonas indica ?
-
?
additional information substrate specificity, no activity with homopolymers of 3-mercaptopropionate and 3-mercaptobutyrate, or the co-polymeric poly(3-hydroxybutyrate-co-3-mercaptopropionate) Pseudomonas indica K2 / DSM 16298 ?
-
?
poly(3-hydroxybutyrate-co-3-mercaptopropionate) + H2O co-polymeric substrate, the activity decreases with increasing amount of 3-mercaptopropionate Schlegelella thermodepolymerans ?
-
?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Schlegelella thermodepolymerans

Synonyms

Synonyms Comment Organism
PHA depolymerase
-
Schlegelella thermodepolymerans
PHA depolymerase
-
Pseudomonas indica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
optimal bacterial growth at 30°C Pseudomonas indica
additional information
-
optimal bacterial growth at 55°C Schlegelella thermodepolymerans
35
-
-
Pseudomonas indica
75 90
-
Schlegelella thermodepolymerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50
-
Pseudomonas indica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
over 90% remaining activity after 24 h Schlegelella thermodepolymerans
70
-
69% remaining activity after 24 h Schlegelella thermodepolymerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Schlegelella thermodepolymerans
8
-
assay at Pseudomonas indica