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Literature summary for 3.1.1.76 extracted from

  • Kim, H.M.; Ryu, K.E.; Bae, K.S.; Rhee, Y.H.
    Purification and characterization of the extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Pseudomonas sp. RY-1 (2000), J. Biosci. Bioeng., 89, 196-198.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by dithioerythritol and phenylmethylsulfonyl fluoride Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
-
Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas sp. utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas sp. RY-1 utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
RY-1
-
Pseudomonas sp. RY-1
-
RY-1
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly[(R)-3-hydroxynonanoate]n + H2O
-
Pseudomonas sp. ?
-
?
poly[(R)-3-hydroxynonanoate]n + H2O
-
Pseudomonas sp. RY-1 ?
-
?
poly[(R)-3-hydroxyoctanoate]n + H2O
-
Pseudomonas sp. poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas sp. poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
poly[(R)-3-hydroxyoctanoate]n + H2O
-
Pseudomonas sp. RY-1 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas sp. RY-1 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Subunits

Subunits Comment Organism
tetramer 4 identical subunits Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas sp.