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Literature summary for 3.1.1.76 extracted from
- Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: putification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13 (1993), Appl. Environ. Microbiol., 59, 1220-1227.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by phenylmethylsulfonyl fluoride | Pseudomonas fluorescens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas fluorescens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration of SDS-treated enzyme | Pseudomonas fluorescens |
| 48000 | - |
gel filtration of native enzyme | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly[(R)-3-hydroxyoctanoate]n + H2O | Pseudomonas fluorescens | utilization of bacterial PHA storage compounds | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | x = 1-5 | ? |  |
| poly[(R)-3-hydroxyoctanoate]n + H2O | Pseudomonas fluorescens GK13 | utilization of bacterial PHA storage compounds | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | x = 1-5 | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
GK13 | - |
| Pseudomonas fluorescens GK13 | - |
GK13 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from culture medium | Pseudomonas fluorescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Pseudomonas fluorescens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fatty acid p-nitrophenyl esters + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| fatty acid p-nitrophenyl esters + H2O | - |
Pseudomonas fluorescens GK13 | ? | - |
? | |
| additional information | completely inactive on poly(3hydroxybutyrate) and other short chain polyhydroxyalkanoates | Pseudomonas fluorescens | ? | - |
? | |
| additional information | completely inactive on poly(3hydroxybutyrate) and other short chain polyhydroxyalkanoates | Pseudomonas fluorescens GK13 | ? | - |
? | |
| poly[(R)-3-hydroxyoctanoate]n + H2O | acts also on copolymers, only with medium chain lenghth compounds | Pseudomonas fluorescens | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | x: 1-5 | ? | |
| poly[(R)-3-hydroxyoctanoate]n + H2O | acts also on copolymers, only with medium chain lenghth compounds | Pseudomonas fluorescens | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | di-3-hydroxyoctanoic acid as main product | ? | |
| poly[(R)-3-hydroxyoctanoate]n + H2O | utilization of bacterial PHA storage compounds | Pseudomonas fluorescens | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | x = 1-5 | ? | |
| poly[(R)-3-hydroxyoctanoate]n + H2O | acts also on copolymers, only with medium chain lenghth compounds | Pseudomonas fluorescens GK13 | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | x: 1-5 | ? | |
| poly[(R)-3-hydroxyoctanoate]n + H2O | acts also on copolymers, only with medium chain lenghth compounds | Pseudomonas fluorescens GK13 | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | di-3-hydroxyoctanoic acid as main product | ? | |
| poly[(R)-3-hydroxyoctanoate]n + H2O | utilization of bacterial PHA storage compounds | Pseudomonas fluorescens GK13 | poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x | x = 1-5 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | gel filtration results of native and SDS-treated enzyme | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 32 | - |
Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 45 | - |
Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.3 | - |
Pseudomonas fluorescens |
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