Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 and phaZ2 (EC 3.1.1.22, UniProt ID Q0K7T2) has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Cupriavidus necator | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0KCI0 | i.e. Alcaligenes eutrophus or Ralstonia eutropha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(R)-3-hydroxybutanoate]n + H2O | x is 1-5 | Cupriavidus necator | [(R)-3-hydroxybutanoate]n-x + [(R)-3-hydroxybutanoate]x | - |
? |
Synonyms | Comment | Organism |
---|---|---|
intracellular poly(3-hydroxybutyrate) depolymerase | UniProt | Cupriavidus necator |
PhaZ1 | - |
Cupriavidus necator |