Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.75 extracted from

  • Lee, M.; Liu, E.; Yang, C.; Hsiao, L.; Wu, T.; Li, S.
    Co-expression of ORFCma with PHB depolymerase (PhaZCma) in Escherichia coli induces efficient whole-cell biodegradation of polyesters (2018), Biotechnol. J., 13, 1700560 .
    View publication on PubMed
Search for more literature for 3.1.1.75

Application

Application Comment Organism
degradation the extra-cellular fraction of Escherichia coli expressing PhaZ exhibits a high poly(3-hydroxybutanoate) degradation rate. It takes 35 h to completely degrade oly(3-hydroxybutanoate) films, while Caldimonas manganoxidans takes 81 h. The coexpression of putative periplasmic substrate binding protein ORFCma further improves the PHB degradation. The enzyme is also able to degrade poly(lactic acid) polycaprolactone, and poly(butylene succinate-co-adipate) Caldimonas manganoxidans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caldimonas manganoxidans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular recombinant enzyme Caldimonas manganoxidans
-
-

Organism

Organism UniProt Comment Textmining
Caldimonas manganoxidans Q9LBN6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(4-hydroxybutyrate)n + H2O
-
 Caldimonas manganoxidans poly(4-hydroxybutyrate)n-x + poly(4-hydroxybutyrate)x
-
 ?
poly(butylene succinate-co-adipate)n + H2O
-
 Caldimonas manganoxidans ?
-
 ?
poly(caprolactone)n + H2O
-
 Caldimonas manganoxidans ?
-
 ?
poly(lactic acid)n + H2O
-
 Caldimonas manganoxidans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, calculated from sequence, x * 40000, SDS-PAGE Caldimonas manganoxidans

Synonyms

Synonyms Comment Organism
PhaZ
-
 Caldimonas manganoxidans