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Literature summary for 3.1.1.75 extracted from

  • Allen, A.; Anderson, W.; Ayorinde, F.; Eribo, B.
    Isolation and characterization of an extracellular thermoalkanophilic P(3HB-co-3HV) depolymerase from Streptomyces sp. IN1 (2011), Int. Biodeterior. Biodegradation, 65, 777-785.
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Streptomyces sp.
DTT
-
 Streptomyces sp.
K+
-
 Streptomyces sp.
Mg2+
-
 Streptomyces sp.
additional information no inhibition of activity by azide Streptomyces sp.
Na+
-
 Streptomyces sp.
PMSF
-
 Streptomyces sp.
Tween 80
-
 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 poly(3-hydroxybutyrate-co-3-hydroxyvalerate) pH 8.0, 30°C Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, about, SDS-PAGE and mass spectrometry Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces sp.
-
 ?
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O Streptomyces sp. IN1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 soil isolate
-
Streptomyces sp. IN1
-
 soil isolate
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 401fold by ammonium sulfate fractionation and gel filtration Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12030
-
 purified native enzyme, substrate poly(3-hydroxybutyrate-co-3-hydroxyvalerate), pH 8.0, 30°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Streptomyces sp. 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Streptomyces sp. IN1 4-nitrophenol + butyrate
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
 Streptomyces sp. ?
-
 ?
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O
-
 Streptomyces sp. IN1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, about, SDS-PAGE and mass spectrometry Streptomyces sp.

Synonyms

Synonyms Comment Organism
P(3HB-co-3HV) depolymerase
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 purified enzyme, 15 min, stable Streptomyces sp.
90
-
 almost complete deactivation of the purified enzyme after 15 min Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
12
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 12 the enzyme is completely inactivated at pH values below pH 7, but shows optimum activity at pH 12 Streptomyces sp.