Cloned (Comment) | Organism |
---|---|
gene phaZSex, expression in Rhodococcus sp. strain T104 as an extracellular active enzyme, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3) | Streptomyces exfoliatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Streptomyces exfoliatus | |
Mn2+ | - |
Streptomyces exfoliatus | |
NaCl | complete inhibition at 3 M | Streptomyces exfoliatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces exfoliatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Streptomyces exfoliatus | |
Mg2+ | activates | Streptomyces exfoliatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(3-hydroxybutyrate) + H2O | Streptomyces exfoliatus | - |
? | - |
? | |
poly(3-hydroxybutyrate) + H2O | Streptomyces exfoliatus DSM Z 41693 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces exfoliatus | - |
gene phaZSex | - |
Streptomyces exfoliatus DSM Z 41693 | - |
gene phaZSex | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Rhodococcus sp. strain T104 cell culture medium to homogeneity | Streptomyces exfoliatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Streptomyces exfoliatus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Streptomyces exfoliatus DSM Z 41693 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Streptomyces exfoliatus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Streptomyces exfoliatus DSM Z 41693 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Streptomyces exfoliatus | 4-nitrophenol + valerate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Streptomyces exfoliatus DSM Z 41693 | 4-nitrophenol + valerate | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces exfoliatus | ? | - |
? | |
poly(3-hydroxybutyrate) + H2O | - |
Streptomyces exfoliatus DSM Z 41693 | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | with 5 or 12 mol% 3-hydroxyvalerate content | Streptomyces exfoliatus | ? | - |
? | |
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | with 5 or 12 mol% 3-hydroxyvalerate content | Streptomyces exfoliatus DSM Z 41693 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHB depolymerase | - |
Streptomyces exfoliatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Streptomyces exfoliatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
unfolding is observed at 55.8°C in a first stage | Streptomyces exfoliatus |
40 | - |
the purified enzyme is highly stable at 40°C | Streptomyces exfoliatus |
70 | - |
the purified enzyme is completely inactivated after 90 min | Streptomyces exfoliatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces exfoliatus |