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Literature summary for 3.1.1.75 extracted from
- Identification and characterization of a novel intracellular poly(3-hydroxybutyrate) depolymerase from Bacillus megaterium (2009), Appl. Environ. Microbiol., 75, 5290-5299.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-tagged fusion protein in Escherichia coli | Priestia megaterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35740 | - |
calculated from cDNA | Priestia megaterium |
| 38000 | - |
His-tagged fusion protein, SDS-PAGE | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | C7A621 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxybutyrate) + H2O | intracellular native poly(3-hydroxybutyrate) granules in vitro are rapidly degraded without the need for protease treatment. Almost all the final hydrolytic products produced from the in vitro degradation of nPHB granules by PhaZ1 are 3-hydroxybutyric acid monomers. PhaZ1 is also able to hydrolyze denatured semicrystalline poly(3-hydroxybutyrate), with the generation of 3-hydroxybutyric acid monomers | Priestia megaterium | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhaZ1 | - |
Priestia megaterium |
| poly(3-hydroxybutyrate) depolymerase | - |
Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Priestia megaterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | disruption of the phaZ1 gene significantly affects intracellular poly(3-hydroxybutyrate) mobilization during the poly(3-hydroxybutyrate)-degrading stage in Bacillus megaterium, as demonstrated by transmission electron microscopy and the measurement of the poly(3-hydroxybutyrate) content | Priestia megaterium |
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Release 2023.1 (January 2023)
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