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Literature summary for 3.1.1.75 extracted from

  • Shah, A.A.; Hasan, F.; Hameed, A.; Ahmed, S.
    A novel poly(3-hydroxybutyrate)-degrading Streptoverticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase (2008), Acta Biol. Hung., 59, 489-499.
    View publication on PubMed
Search for more literature for 3.1.1.75

Application

Application Comment Organism
biotechnology the optimum production of PHB depolymerase is observed at pH 8 and 7, at 45°C, 1% substrate concentration and in the presence of lactose as an additional carbon source Streptomyces kashimirensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 SDS-PAGE shows two bands Streptomyces kashimirensis
45000
-
 SDS-PAGE shows two bands Streptomyces kashimirensis

Organism

Organism UniProt Comment Textmining
Streptomyces kashimirensis
-
 AF1
-
Streptomyces kashimirensis AF1
-
 AF1
-

Purification (Commentary)

Purification (Comment) Organism
PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75 Streptomyces kashimirensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly (3-hydroxybutyrate) + H2O
-
 Streptomyces kashimirensis ?
-
 ?
poly (3-hydroxybutyrate) + H2O
-
 Streptomyces kashimirensis AF1 ?
-
 ?

Synonyms

Synonyms Comment Organism
PHB-depolymerase
-
 Streptomyces kashimirensis
poly(3-hydroxybutyrate) depolymerase
-
 Streptomyces kashimirensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces kashimirensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces kashimirensis