Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM, 32% loss of activity | Penicillium sp. | |
Ca2+ | 5 mM, 44% loss of activity | Penicillium sp. | |
Cu2+ | 5 mM, complete loss of activity | Penicillium sp. | |
Fe2+ | 5 mM, 50% loss of activity | Penicillium sp. | |
Fe3+ | 5 mM, 38% loss of activity | Penicillium sp. | |
Hg2+ | 5 mM, 97% loss of activity | Penicillium sp. | |
K+ | 5 mM, 32% loss of activity | Penicillium sp. | |
Na+ | 5 mM, 10% loss of activity | Penicillium sp. | |
Zn2+ | 5 mM, 88% loss of activity | Penicillium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Penicillium sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 1.1fold activation | Penicillium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15100 | - |
x * 15100, SDS-PAGE | Penicillium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium sp. | - |
DS9713a-01 | - |
Penicillium sp. DS9713a-01 | - |
DS9713a-01 | - |
Purification (Comment) | Organism |
---|---|
- |
Penicillium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | the activity of the extracellular PHB depolymerase reaches a maximum at 72 h when it grows in the medium containing poly (3-hydroxybutyrate) as the sole carbon source | Penicillium sp. | - |
Storage Stability | Organism |
---|---|
-20°C, 5 mM Na2HPO4KH2PO4 pH 8.6, stable for about 1 year | Penicillium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly[(R)-3-hydroxybutanoate]n + H2O | activity of mutant strain DS9713a-01 obtained by ultraviolet light mutagenesis is 97% higher than that of the wild-type strain. The DS9713a-01 mutant can completely degrade PHB films in 5 days. The wild-type strain achieves only 61% at the same time. The primary product is the monomer, 3-hydroxybutyric acid | Penicillium sp. | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
poly[(R)-3-hydroxybutanoate]n + H2O | activity of mutant strain DS9713a-01 obtained by ultraviolet light mutagenesis is 97% higher than that of the wild-type strain. The DS9713a-01 mutant can completely degrade PHB films in 5 days. The wild-type strain achieves only 61% at the same time. The primary product is the monomer, 3-hydroxybutyric acid | Penicillium sp. DS9713a-01 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15100, SDS-PAGE | Penicillium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable below | Penicillium sp. |
40 | - |
2 h, 39% loss of activcity | Penicillium sp. |
55 | - |
2 h complete loss of activity | Penicillium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Penicillium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 40% of maximal activity, pH 10.0: about 30% of maximal activity | Penicillium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9.2 | 40°C, 2 h, stable | Penicillium sp. |