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Literature summary for 3.1.1.75 extracted from

  • Ci, S.; Shan, C.; Liu, D.; Xia, H.
    An extracellular poly(3-hydroxybutyrate) depolymerase from Penicillium sp. DS9713a-01 (2006), World J. Microbiol. Biotechnol., 22, 729-735.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM, 32% loss of activity Penicillium sp.
Ca2+ 5 mM, 44% loss of activity Penicillium sp.
Cu2+ 5 mM, complete loss of activity Penicillium sp.
Fe2+ 5 mM, 50% loss of activity Penicillium sp.
Fe3+ 5 mM, 38% loss of activity Penicillium sp.
Hg2+ 5 mM, 97% loss of activity Penicillium sp.
K+ 5 mM, 32% loss of activity Penicillium sp.
Na+ 5 mM, 10% loss of activity Penicillium sp.
Zn2+ 5 mM, 88% loss of activity Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Penicillium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM, 1.1fold activation Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15100
-
x * 15100, SDS-PAGE Penicillium sp.

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
DS9713a-01
-
Penicillium sp. DS9713a-01
-
DS9713a-01
-

Purification (Commentary)

Purification (Comment) Organism
-
Penicillium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium the activity of the extracellular PHB depolymerase reaches a maximum at 72 h when it grows in the medium containing poly (3-hydroxybutyrate) as the sole carbon source Penicillium sp.
-

Storage Stability

Storage Stability Organism
-20°C, 5 mM Na2HPO4–KH2PO4 pH 8.6, stable for about 1 year Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O activity of mutant strain DS9713a-01 obtained by ultraviolet light mutagenesis is 97% higher than that of the wild-type strain. The DS9713a-01 mutant can completely degrade PHB films in 5 days. The wild-type strain achieves only 61% at the same time. The primary product is the monomer, 3-hydroxybutyric acid Penicillium sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?
poly[(R)-3-hydroxybutanoate]n + H2O activity of mutant strain DS9713a-01 obtained by ultraviolet light mutagenesis is 97% higher than that of the wild-type strain. The DS9713a-01 mutant can completely degrade PHB films in 5 days. The wild-type strain achieves only 61% at the same time. The primary product is the monomer, 3-hydroxybutyric acid Penicillium sp. DS9713a-01 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
?

Subunits

Subunits Comment Organism
? x * 15100, SDS-PAGE Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable below Penicillium sp.
40
-
2 h, 39% loss of activcity Penicillium sp.
55
-
2 h complete loss of activity Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
Penicillium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 40% of maximal activity, pH 10.0: about 30% of maximal activity Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 9.2 40°C, 2 h, stable Penicillium sp.