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Literature summary for 3.1.1.75 extracted from

  • Nojima, S.; Mineki, S.; Iida, M.
    Purification and characterization of extracellular poly(3-hydroxybutyrate) depolymerases produced by Agrobacterium sp. K-03 (1996), J. Ferment. Bioeng., 81, 72-75.
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol considerable inactivation Agrobacterium sp.
phenylmethanesulfonyl fluoride considerable inactivation Agrobacterium sp.
Tween 20 considerable inactivation Agrobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 poly(3-hydroxybutanoate)n 0.018 mg/ml, isoform E1, 0.07 mg/ml, isoform E2 Agrobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Agrobacterium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 isofrom E2, gel filtration Agrobacterium sp.
46000
-
 isoform E1, gel filtration Agrobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Agrobacterium sp.
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Agrobacterium sp. K-03
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium sp.
-
-
-
Agrobacterium sp. K-03
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2 isoforms, E1, E2 Agrobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Agrobacterium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Agrobacterium sp. (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Agrobacterium sp. K-03 (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n + H2O
-
 Agrobacterium sp. poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)
-
 ?
poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n + H2O
-
 Agrobacterium sp. K-03 poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)
-
 ?
poly(3-hydroxybutyric acid) + H2O
-
 Agrobacterium sp. ?
-
 ?
poly(3-hydroxybutyric acid) + H2O
-
 Agrobacterium sp. K-03 ?
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Agrobacterium sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Agrobacterium sp. K-03 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Agrobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 isoform E2 Agrobacterium sp.
8.1
-
 isoform E1 Agrobacterium sp.