Literature summary for 3.1.1.75 extracted from

Sadacco, P.; Nocerino, S.; Dubini-Paglia, E.; Seves, A.; Elegir, G.
Characterization of a poly(3-hydroxybutyrate) depolymerase from Aureobacterium saperdae: Acive site and kinetics of hydrolysis studies (1997), J. Environ. Polym. Degrad., 5, 57-65.

No PubMed abstract available

Search for more literature for 3.1.1.75

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Hydroxy-5-nitrobenzyl bromide	complete inactivation	Microbacterium saperdae
diazo-DL-norleucine methyl ester	very sensitive to	Microbacterium saperdae
diisopropyl fluorophosphate	very sensitive to	Microbacterium saperdae
dithiothreitol	rapid inactivation	Microbacterium saperdae
N-bromosuccinimide	complete inactivation	Microbacterium saperdae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Microbacterium saperdae	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42700	-	gel filtration	Microbacterium saperdae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
poly[(R)-3-hydroxybutanoate]n + H2O	Microbacterium saperdae	-	poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x	main products: dimer and monomer	?

Organism

Organism	UniProt	Comment	Textmining
Microbacterium saperdae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, chromatography steps	Microbacterium saperdae

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Microbacterium saperdae	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly[(R)-3-hydroxybutanoate]n + H2O	-	Microbacterium saperdae	poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x	main products: dimer and monomer	?

Subunits

Subunits	Comment	Organism
monomer	1 * 42700, SDS-PAGE	Microbacterium saperdae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Microbacterium saperdae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	11	stable between	Microbacterium saperdae

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Release 2023.1 (January 2023)